1. Nhazi
A na-ekewa Gel electrophoresis n'ụdị vetikal (gụnyere gels kọlụm na gels slab) na ụdị kwụ ọtọ (karịsịa gels slab) (Nyocha 6-18). N'ozuzu, nkewa kwụ ọtọ dịtụ elu karịa kehoraizin, mana nkwadebe gel kwụ ọtọ nwere ma ọ dịkarịa ala uru anọ: enwere nkwado n'okpuru gel dum, na-enye ohere iji agarose dị ala; ọ ga-ekwe omume ịkwadebe efere gel agarose nke nkọwa dị iche iche; nkwadebe gel na ntinye ihe nlele na-adaba adaba; Ụlọ electrophoresis dị mfe iji wuo na ọnụ ahịa. Ebe ọ bụ na a na-eme electrophoresis kwụ ọtọ na efere agarose na-emikpu n'ụzọ zuru ezu ihe dịka 1mm n'okpuru elu nke electrophoresis buffer, a na-akpọkwa ya electrophoresis submerged.
Electrophoresis Tank DYCP-31DN maka agarose gel electrophoresis
2.Buffer Sistemu
Na nkewa nke nucleic acid, ọtụtụ sistemụ na-agbaso usoro na-aga n'ihu. Ihe nchekwa electrophoresis a na-ejikarị gụnyere TBE (0.08mol/L Tris·HCl, pH 8.5, 0.08mol/L boric acid, 0.0024mol/L EDTA) ihe nchekwa na THE (0.04mol/L Tris·HCl, pH 7.8, 0.02mol/L). sodium acetate, 0.0018mol/L EDTA) ihe nchekwa. A na-akwadokarị ihe nchekwa ndị a dị ka ihe ngwọta ngwaahịa 10x ma tụba ya na ntinye achọrọ mgbe ejiri ya. Ọnụego mbata nke DNA linear na okirikiri dị na agarose gel dị iche na ihe nchekwa ejiri. N'ime ihe nchekwa ahụ, ọnụọgụ DNA linear na-akwaga karịa nke DNA okirikiri, ebe na nchekwa TBE, ihe dị iche bụ eziokwu.
3.Nkwadebe nke Agarose Gel
(1) Nkwadebe nke Horizontal Agarose Gel
(a) Kwadebe ntinye uche nke agarose gel na-eji 1x electrophoresis buffer.
(b) Kpoo agarose ka ọ gbazee ma ọ bụ n'ime mmiri ịsa mmiri na-esi esi, na ihe ndọta magnetik, ma ọ bụ na ngwa ndakwa nri. Mee ka ngwọta agarose dị jụụ ruo 55 Celsius ma gbakwunye ethidium bromide (EB) agba na njedebe ikpeazụ nke 0.5 μg / ml.
(c) Mechie n'ọnụ nke iko ma ọ bụ acrylic efere na obere ego nke agarose gel, tinye a mbo, na-dobe comb ezé banyere 0.5 ~ 1.0 mm n'elu efere.
(d) Wunye ihe ngwọta agarose gbazere na-aga n'ihu n'ime iko ma ọ bụ acrylic plate (okpukpu na-adabere na olu nke ihe nlele DNA), na-ezere iwebata ikuku ikuku. Mee ka o sie ike n'okike na ụlọ okpomọkụ.
(e) Jiri nlezianya wepụ mbo ahụ mgbe ọ gbasiri ike. Tinye ihe nkwụnye electrophoresis kwesịrị ekwesị na tank gel, na-ahụ na efere gel na-emikpu ihe dị ka 1 mm n'okpuru elu nke electrophoresis buffer.
(2) Nkwadebe nke Gel Agarose kwụ ọtọ
(a) Wepu griiz ma ọ bụ ihe fọdụrụ na efere iko site na iji ethanol saa.
(b) Debe efere spacer n'etiti dams n'ihu na azụ, dozie nsọtụ nke efere spacer na dams ihu na azụ, ma chekwaa ha na mkpọ.
(c) Tinye 2% agarose na nchekwa 1x n'etiti nsọtụ nke efere spacer iji mepụta plọse agarose dị elu 1 cm na ala nke ụlọ nkedo gel.
(d) Wunye gel agarose gbazere na ntinye uche achọrọ, kwadebere na nchekwa 1x, n'ime ụlọ gel ruo 1 cm n'okpuru elu.
(e) Fanye mbo, na-ezere ọnyà ikuku n'okpuru ezé mbo. Mgbe ụfọdụ, wrinkles nwere ike ịpụta na mbo eze n'oge agarose gel jụrụ; N'ọnọdụ ndị dị otú a, tinye naanị agarose gbazere n'elu ka ọ sie ike.
(f) Wepu mbo. Iji gbochie ntapu ihe nchekwa na oghere ntinye, mechie njikọ dị n'etiti efere agarose gel na ụlọ electrophoresis na 2% agarose ma gbakwunye ego nchekwa achọrọ.
(g) Tinye ihe nchekwa electrophoresis 1x na ọnụ ụlọ gel.
(h) Jiri nlezianya buru ihe nlele DNA na gel agarose dị n'okpuru ebe nchekwa ahụ.
Ozi ndị ọzọ gbasara ihe ọmụma bụ isi banyere agarose gel electrophoresis, anyị ga-ekekọrịta n'izu na-abịa. Achọrọ ka ozi ndị a nyere aka na nnwale gị.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Isi ngwaahịa anyị bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Anyị na-achọ ugbu a ndị mmekọ, ma OEM electrophoresis tank na nkesa na-anabata.
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Oge nzipu: Dec-07-2023