Usoro ntọala nke Agarose Gel Electrophoresis(2)

Nkwadebe ihe atụ naNa-ebugo

N'ihi iji usoro nchekwa na-aga n'ihu na-enweghị stacking gel n'ọtụtụ ọnọdụ, ihe nlele ahụ kwesịrị inwe ntinye kwesịrị ekwesị na obere olu. Jiri apipetteiji jiri nwayọọ tinye ihe nlele ahụ, na 5-10 μg kwa nke ọma, iji zere mbelata dị ukwuu na mkpebi. Mgbe olenbudata sample, ọkụ ọkụ ga-agbanyụrịrị. Ihe nlele ahụ kwesịrị ịnwe ihe ngosi agba (0.025% bromophenol blue ma ọ bụ oroma) na sucrose (10-15%) ma ọ bụ glycerol (5-10%) iji mee ka njupụta ya dịkwuo elu, tinye uche na nlele ahụ, ma gbochie mgbasa. Otú ọ dị, mgbe ụfọdụ sucrose ma ọ bụ glycerol nwere ike ime ka eriri U-ụdị na nsonaazụ electrophoresis, nke enwere ike izere site na iji 2.5% Ficoll (polyvinylpyrrolidone).

Electrophoresis

Voltaji maka electrophoresis bụ 5-15 V / cm, n'ozuzu gburugburu 10 V / cm. Maka nkewa nke nnukwu ụmụ irighiri ihe, voltaji kwesịrị ịdị ala, na-adịkarịghị karịa 5 V / cm.

Ntucha

Fluorescent dye ethidium bromide (EB) bụ nke a na-ejikarị eme ihe maka ntụpọ iji hụ eriri DNA na gel agarose. EB nwere ike itinye n'etiti ụzọ abụọ isi nke mkpụrụ ndụ DNA, na-eme ka EB jikọọ na DNA. A na-ebufe mmịpụta nke 260 nm ultraviolet ìhè site na DNA na EB, na EB na-ejikọta ya na-ebute fluorescence na 590 nm na mpaghara uhie-oroma nke ụdị ọkụ a na-ahụ anya. Ịmepụta gel na ngwọta 1 mmol/L MgSO4 maka awa 1 nwere ike ibelata ọkụ ọkụ n'azụ nke EB na-adịghị ejikọta, na-eme ka nchọpụta nke obere DNA.

EB dye nwere ọtụtụ uru: ọ dị mfe iji, adịghị agbaji nucleic acid, nwere mmetụta dị elu, ma nwee ike imebi ma DNA na RNA. Enwere ike ịgbakwunye EB na nlele ma soro ya site na iji absorption UV n'oge ọ bụla. Mgbe agbachara, EB nwere ike wepu ya site na mmịpụta na n-butanol.

Agbanyeghị, agba EB bụ mutagen nwere ike, yana ịkpachara anya, dị ka iyi uwe polyethylene, kwesịrị iwere n'oge ejizi ya. Acridine oroma bụkwa ihe a na-ejikarị esiji eme ihe n'ihi na ọ nwere ike ịmata ọdịiche dị n'etiti acid nucleic acid nke nwere eriri na abụọ (DNA, RNA). Ọ na-egosiputa fluorescence green (530 nm) maka acid nucleic nwere eriri abụọ na fluorescence na-acha uhie uhie (640 nm) maka acid nucleic nwere otu eriri. Na mgbakwunye, enwere ike iji agba agba ndị ọzọ dị ka methylene blue, methylene green, na quinoline B mee ihe maka nsị.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Isi ngwaahịa anyị bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.

Anyị na-achọ ugbu a ndị mmekọ, ma OEM electrophoresis tank na nkesa na-anabata.

Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị. Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.

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