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Comet Assay: Usoro nwere mmetụta maka ịchọpụta mmebi na mmezi DNA

Comet Assay (Single Cell Gel Electrophoresis, SCGE) bụ usoro na-emetụ n'ahụ na ngwa ngwa nke a na-ejikarị achọpụta mmebi na nrụzi DNA na sel nke ọ bụla. Aha "Comet Assay" sitere na njirimara ọdịdị nke comet-dị ka nke na-apụta na nsonaazụ ya: nucleus nke cell na-eme "isi," ebe iberibe DNA mebiri emebi na-akwaga, na-emepụta "ọdụ" yiri comet.

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Ụkpụrụ

Ụkpụrụ nke Comet Assay dabeere na mbugharị nke iberibe DNA na mpaghara eletrik. DNA na-edoghị anya na-anọgide n'ime oghere cell, ebe DNA mebiri emebi ma ọ bụ nke kewara ekewa na-akwaga na anode, na-akpụ "ọdụ" comet. Ogologo na ike nke ọdụ ahụ dabara kpọmkwem na oke mmebi DNA.

Usoro

  1. Nkwadebe cell: A na-agwakọta mkpụrụ ndụ ndị a ga-anwale na agarose na-agbaze dị ala ma gbasaa na slide microscope ka ọ bụrụ otu oyi akwa.
  2. Cell Lysis: A na-emikpu ihe mmịfe ahụ n'ime ngwọta lysis iji wepụ akpụkpọ ahụ cell na akpụkpọ ahụ nuklia, na-ekpughe DNA.
  3. Electrophoresis: A na-etinye ihe mmịfe ahụ n'ime ụlọ electrophoresis n'okpuru ọnọdụ alkaline ma ọ bụ na-anọpụ iche. Iberibe DNA mebiri emebi na-akwaga na electrode dị mma n'okpuru mmetụta nke mpaghara eletrik.
  4. Ntucha: Mgbe electrophoresis gasịrị, a na-eji ihe na-acha fluorescent (dịka ethidium bromide) mee ihe slide ndị ahụ iji hụ anya DNA.
  5. Nyocha microscopic: Iji microscope fluorescence ma ọ bụ sọftụwia pụrụ iche, a na-enyocha ụdị comet ahụ, a na-atụkwa ihe ndị dị ka ogologo ọdụ na ike.

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Foto sitere na biorender

Nyocha data

A na-enyocha nsonaazụ sitere na Comet Assay dabere n'ọtụtụ isi ihe:

  • Ogologo Ogologo: Na-anọchite anya anya DNA na-akwaga, na-egosi oke mmebi DNA.
  • Ọdịnaya DNA ọdụ: Pasent DNA nke na-akwaga na ọdụ, a na-ejikarị eme ihe dị ka ọnụọgụ ọnụọgụ DNA mebiri.
  • Oge ọdụ Olive (OTM): Na-ejikọta ma ogologo ọdụ na ọdịnaya DNA ọdụ iji nye nkọwa zuru oke nke mmebi DNA.

Ngwa

  1. Ọmụmụ Genotoxicity: A na-eji Comet Assay eme ihe n'ọtụtụ ebe iji nyochaa mmetụta nke kemịkalụ, ọgwụ, na radieshon na DNA cell, na-eme ka ọ bụrụ ihe dị mkpa maka nyocha mkpụrụ ndụ ihe nketa.
  2. Environmental Toxicology: Ọ na-enyere aka n'inyocha mmetụta nke mmetọ gburugburu ebe obibi na DNA nke ihe ndị dị ndụ, na-enye nghọta na nchekwa gburugburu ebe obibi.
  3. Nnyocha ahụike na ahụike: A na-eji Comet Assay na-amụ usoro nrụzi DNA, kansa, na ọrịa ndị ọzọ metụtara DNA. Ọ na-enyochakwa mmetụta ọgwụgwọ ọrịa kansa dị ka radiotherapy na chemotherapy na DNA.
  4. Sayensị nri na ọrụ ugbo: A na-eji ya nyochaa nchekwa nke ọgwụ pesticides, ihe mgbakwunye nri, na ihe ndị ọzọ, yana nyocha mmetụta nsị ha na ụdị anụmanụ.

Uru

  • Mmetụta dị elu: Nwere ike ịchọpụta ọkwa dị ala nke mmebi DNA.
  • Arụ Ọrụ Dị Mfe: Usoro ahụ kwụ ọtọ, na-eme ka ọ dị mma maka nyocha dị elu.
  • Ngwa sara mbara: Enwere ike itinye ya na ụdị sel dị iche iche, gụnyere mkpụrụ ndụ anụmanụ na osisi.
  • Ihe ịma aka nha nha: Mgbe ị na-enye data qualitative na mmebi DNA, nyocha ọnụọgụ na-adabere kpamkpam na ngwanrọ na usoro nyocha ihe oyiyi.
  • Ọnọdụ nnwale: Nsonaazụ nwere ike imetụta ihe ndị dị ka oge electrophoresis na pH, na-achọ njikwa nlezianya nke ọnọdụ nnwale.

Oke

Comet Assay bụ ngwá ọrụ bara uru na nyocha biomedical, sayensị gburugburu ebe obibi, na mmepe ọgwụ n'ihi mgbanwe ya na nghọta dị elu n'ịchọpụta mmebi na nrụzi DNA. Ụgwọ nke ụlọ ọrụ Beijing Liuyi Biotechnology Co.,Ltd (Liuyi Biotechnology)na-enye ụlọ electrophoresis kwụ ọtọ maka nyocha comet. Nabata ịkpọtụrụ anyị ka anyị kparịta banyereComet Assayprotocol.

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Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.

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Oge nzipu: Sep-20-2024