Ihe gbasara Electrophoresis DNA

Gel electrophoresis bụ otu n'ime ụzọ ndị bụ isi a na-eji na bayoloji molecular maka nyocha nke DNA. Usoro a gụnyere ịkwaga iberibe DNA site na gel, ebe a na-ekewa ha dabere na nha ma ọ bụ ọdịdị. Otú ọ dị, ọ dịtụla mgbe ị zutere njehie ọ bụla n'oge nyocha electrophoresis gị, dị ka ụgbụ a na-agbanye na gel agarose, ma ọ bụ enweghị eriri na gel? Gịnị nwere ike ịbụ ihe kpatara njehie ndị a?

na-akpasu iwe

Ndị ọrụ nka anyị achịkọtala ndị di na nwunye nke nchọpụta nsogbu ebe a maka ntụaka gị.

1. Akpụkpọ anụ na agarose gel

smeard bandeji na gel

DNA e wedara. Zere mmetọ nuclease.

● Ihe nchekwa electrophoresis adịchaghị ọhụrụ. Mgbe ejiri electrophoresis eme ihe ugboro ugboro, ike ionic na-ebelata, uru pH ya na-abawanye, ya mere ikike nchekwa ahụ na-ebelata, nke na-emetụta mmetụta electrophoresis. A na-atụ aro ka dochie ihe nchekwa electrophoresis ugboro ugboro.

● A na-eji ọnọdụ electrophoresis na-ezighị ezi mee ihe. Ekwela ka voltaji gafere 20 V/cm, ma nọgide na-enwe okpomọkụ <30°C n'oge electrophoresis. Maka nnukwu DNA strand electrophoresis, okpomọkụ kwesịrị ịdị <15 Celsius C. Lelee ihe nchekwa electrophoresis nwere ikike nchekwa zuru oke.

● A na-etinye DNA nke ukwuu na gel. Belata ọnụọgụ DNA.

● Nnukwu nnu na DNA. Jiri mmiri ozuzo ethanol wepụ oke nnu n'oge dị elu.

● E ji protein metọọ DNA. Jiri mmịpụta phenol wepụ protein dị elu.

● A pụnara DNA ahụ. Ejila ọkụ tupu electrophoresis. Jiri 20 mM NaCl gbanye DNA na nchekwa.

2. Anomalie DNA band migration

● Mweghachi nke saịtị COS nke λHind III iberibe. Kpoo DNA maka nkeji 5 n'okpuru 65 Celsius C tupu electrophoresis, wee kpoo ya na ice unit maka nkeji ise.

● A na-eji ọnọdụ electrophoresis na-ezighị ezi mee ihe. Ekwela ka voltaji gafere 20 V/cm, ma nọgide na-enwe okpomọkụ <30°C n'oge electrophoresis. Lelee ihe nchekwa electrophoresis nwere ikike nchekwa zuru oke.

● A pụnara DNA ahụ. Ejila ọkụ tupu electrophoresis. Jiri 20 mM NaCl gbanye DNA na nchekwa.

3. Na-ada mbà ma ọ bụ enweghị DNA bandeji na agarose gel

ụgbụ DNA na-adịghị ala ala

● DNA enweghị oke ma ọ bụ itinye uche zuru oke na gel. Mee ka ọnụọgụ DNA dịkwuo elu. Polyacrylamide gel electrophoresis dị ntakịrị mmetụta karịa agarose electrophoresis, enwere ike ibelata ntinye nlele nke ọma.

● DNA e wedara ala. Zere mmetọ nuclease.

● A na-ewepụ DNA ahụ site na gel. Electrophorese gel maka obere oge, jiri obere voltaji, ma ọ bụ jiri gel pasent dị elu.

● A na-eji isi iyi W na-ezighi ezi mee ka anya nke DNA nwere ethidium bromide. Jiri ọkụ dị mkpụmkpụ (254 nm) W maka nghọta ka ukwuu.

4. DNA bandeji efu

DNA dị ntakịrị ka agbapụrụ na gel. Electrophorese gel maka obere oge, jiri obere voltaji, ma ọ bụ jiri gel pasent dị elu.

● Ọ na-esiri ike ịmata ọdịiche nke eriri DNA nke ihe ndị yiri ya. Mee ka oge electrophoresis dịkwuo elu, ma lelee ntinye uchenke gel iji jide n'aka na pasent gel nke ọma ga-eji.

● A pụnara DNA ahụ. Ejila ọkụ tupu electrophoresis. Jiri 20 mM NaCl gbanye DNA na nchekwa.

● Eriri DNA buru ibu, gel electrophoresis a na-emekarị adịghịkwa mma. Nyochaa na pulse gel electrophoresis.Kedu nsogbu ndị ọzọ ị nwere na agarose gel electrophoresis? Anyị ga-enyochakwu maka ntuziaka n'ọdịnihu.

Beijing Liuyi biotechnology Co., Ltd (Liuyi Biotech) bụ ụlọ ọrụ pụrụ iche na-elekwasị anya na ngwaahịa ndị metụtara electrophoresis na China. Akụkọ ya malitere na 1970 mgbe China abanyebeghị na mgbanwe na oge mmeghe. Site na mmepe nke afọ, Liuyi Bitotech nwere akara nke ya, nke a maara dị ka Liuyi Brand n'ahịa ụlọ maka ngwaahịa electrophoresis.

Ụdị Liuyi nwere ihe karịrị afọ 50 akụkọ ihe mere eme na China na ụlọ ọrụ ahụ nwere ike ịnye ngwaahịa kwụsiri ike na nke dị elu gburugburu ụwa. Site na mmepe nke afọ, ọ dabara nke nhọrọ gị!

Mkpụrụ ndụ electrophoresis kwụ ọtọ (tankị / ụlọ) nke Liuyi Biotech dị oke mma na ọdịdị dị mma. Site na nha dị iche iche nke trays gel, ha nwere ike izute ihe nnwale nnwale gị dị iche iche. Anyị nwere otu ọrụ nka na ụlọ ọrụ mmepụta ihe. Site na imewe iji rụpụta ihe, ihe akụrụngwa na akụkụ ndị bụ isi, anyị nwere ike ijikwa usoro niile. Usoro DYCP 31 bụ maka DNA electrophoresis, nke bụ ihe nlereanyaDYCP-31BN, DYCP-31CN,DYCP-31DN, naDYCP-31E. Ihe dị iche n'etiti ha bụ nha gel na ọnụahịa. Anyị na-enye ngwaahịa zuru oke maka ndị ahịa anyị. Ihe nlereanyaDYCP-32Cnwere ike ime ka ndị kasị ibu jel 250mm * 250mm.

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Ka ọ dịgodị, anyị na-akwado anyị electrophoresis ike ọkọnọMMỤỌ-6C,MMỤỌ-6DnaAfọ-10Cmaka sel electrophoresis anyị (tankị / ụlọ) DYCP-31 na usoro 32.

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Oge nzipu: Mee-09-2022