DNA Gel Electrophoresis: Nyochaa iberibe mkpụrụ ndụ ihe nketa

Gel electrophoresis DNA bụ usoro ihe ọmụmụ ihe omimi nke a na-ejikarị maka ikewa na nyochaa iberibe DNA dabere na nha ha. Usoro a gụnyere ibunye iberibe DNA dị iche iche n'elu gel nke agarose, bụ́ carbohydrate dị na algae na-acha uhie uhie.

Na-akwadebe ma na-atụgharị gel agarose

1. Gbanyụọ agarose n'ụdị ihe nchekwa electrophoresis kwesịrị ekwesị. A na-ekpebi ntinye nke gel site na oke-na-olu, dị ka 1g nke agarose na 100.ml ihe nchekwa maka 1% gel.
2. Kpoo ngwakọta a na ngwa ndakwa nri, na-atụgharị akpa ahụ iji hụ na mgbasa nke agarose zuru ezu.
3. Tinye ethidium bromide na ngwọta gel na ntinye ikpeazụ nke 0,5mg/ ml. Ethidium bromide gbakọrọ n'etiti ụzọ abụọ DNA dị n'akụkụ wee na-ewepụta fluorescence oroma n'okpuru ọkụ UV. Mara na ethidium bromide bụ ọrịa kansa, yabụ ijikwa ya chọrọ usoro nchekwa kwesịrị ekwesị dị ka iyi uwe aka.
4. Mee ka ngwọta gel dị jụụ n'ime mmiri ịsa ahụ iji gbochie tray gel ka ọ ghara ịdaba n'ihi oke okpomọkụ.
5. Tinye mbo n'ime ihe ngwọta jel iji mepụta olulu mmiri nlele. Họrọ combs dabara maka ọnụọgụ DNA ị ga-ebunye. Wunye ngwọta agarose gel n'imejel trayma kwe ka o sie ike na okpomọkụ ụlọ.
6. Ozugbo gel siri ike, wepụ mbo. Ọ bụrụ na ị gaghị eji gel ozugbo, kechie ya na mkpuchi plastik ma chekwaa ya na 4 ℃ ruo mgbe achọrọ ya.

Na-akwadebe ma na-agba ọsọ gel

Tupu ịmalite electrophoresis, gwakọta ihe nlele DNA na ihe nkpuchi. Ihe nchekwa ihe na-ebunye ihe na-etinyekarị uche ugboro isii ma na-enyere ihe nlele ahụ aka n'ala ala olulu mmiri ma soro mmegharị ahụ n'oge electrophoresis.

Tọọ ọkụ ọkụ na voltaji akọwapụtara.

Tinye ihe nchekwa electrophoresis zuru oke na tank gel iji kpuchie elu gel.Gbaa mbọ hụnjikọ electrode ziri ezi.

Bunye ihe nlere DNA na ihe nrịbama arọ molekụla n'ime olulu mmiri jel.

Gbanyụọ ọkụ ka ọ malite electrophoresis.

Na-elele iberibe DNA kewapụrụ iche

Mgbe electrophoresis gasịrị, gbanyụọ ọkụ ma wepụ gel.

Jiri isi iyi ọkụ UV mee ka gel na-enwu; Iberibe DNA ga-apụta dị ka eriri fluorescent oroma.

Detuo ihe oyiyi jel iji nyochaa iberibe DNA ndị kewapụrụ.

Mgbe nnwale ahụ gasịrị, hụ na mkpofu nke ọma nke gel na electrophoresis buffer, na-agbaso ntuziaka ụlọ nyocha. Na-eyi gloves mgbe niile mgbe ị na-eji gels na buffers nwere ethidium bromide iji chebe pụọ na ikpughe ihe a dị ize ndụ.

A na-eji DNA gel electrophoresis eme ihe n'ọtụtụ ebe na usoro ihe ọmụmụ ihe omimi na nyocha mkpụrụ ndụ ihe nketa maka ịkọ nha mkpụrụ ndụ DNA, ikewa iberibe DNA, ịchọpụta mmụgharị mkpụrụ ndụ ihe nketa, na mkpịsị mkpịsị aka DNA, n'etiti ngwa ndị ọzọ. Ọ bụ usoro nnwale dị mfe ma dị irè nke na-enyere aka n'ịghọta nhazi na njirimara nke ihe nlele DNA.

Beijing Liuyi Biotechnology Co.Ltd, a ọkachamara emeputa na-elekwasị anya na electrophoresis ngwaahịa n'elu 50 afọ, awade a nso nke electrophoresis tankị (ụlọ / cell) maka jel electrophoresis, dị ka kehoraizin electrophoresis tank (ụlọ / cell) makaDNAgel electrophoresis, na tankị electrophoresis kwụ ọtọ (ụlọ / cell) maka protein gel electrophoresis. Ka ọ dị ugbu a, ọ na-enyekwa ụdị ọkụ eletrik dị iche iche, onye nyocha igbe igbe ọchịchịrị na UV transilluminator. Ị nwere ike ịhọrọ tankị electrophoresis nke Beijing Liuyi (ụlọ / cell) iji tụọ ịdị arọ nke RNA na ikewapụ RNA nke nha dị iche iche maka ụlọ nyocha gị.

Ebe a anyị ga-kwadorootu ụdị kehoraizin electrophoresis tank (ụlọ / cell) nlereanyaDYCP-31DN ime ma na-agba ọsọ gel.

Mgbe ịmechara gel, ị nwere ike iji ihe oyiyi gel anyị & usoro nyocha usoroWD-9413Biji lelee, nyochaa na see foto maka gel. Liuyi Biotech na-enyekwa UV transilluminator (UV Analyzer) ịhụ gel. Anyị nwere ụdị igbe ojii UV transilluminator (UV Analyzer) ụdịWD-9403A,9403C,WD-9403F, obere UV transilluminator (UV Analyzer) nlereanyaWD-9403Bna aka UV transilluminator (UV Analyzer)WD-9403En'ihi na ị na-ahọrọ.

The Beijing Liuyi ika nwere ihe karịrị 50-afọ akụkọ ihe mere eme na China na ụlọ ọrụ nwere ike na-enye kwụsiri ike na elu-edu ngwaahịa gburugburu ụwa. Site na afọ nke mmepe, ọ bụ ihe kwesịrị ekwesị nke nhọrọ gị!

Anyị na-achọ ugbu a ndị mmekọ, ma OEM electrophoresis tank na nkesa na-anabata.

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