Ụkpụrụ
Cellulose acetate film electrophoresis bụ usoro electrophoresis na-eji ihe nkiri acetate cellulose dị ka nkwado. Ihe a na-eme nke ụmụ irighiri ihe na-ebufe na-aga n'ihu electrode nke ọzọ n'okpuru ọrụ eletrik ka a na-akpọ electrophoresis. Ebe ọ bụ na protein ọ bụla nwere ebe isoelectric akọwapụtara, ọ bụrụ na etinye protein na ngwọta na pH dị ala karịa ebe isoelectric ya, a ga-ebuba protein ahụ nke ọma wee gaa na electrode na-adịghị mma. N'ụzọ megidere nke ahụ, ọ na-akwaga na ogwe osisi dị mma. N'ihi na ọsọ nke ụmụ irighiri protein na-akpụ akpụ na ọkụ eletrik metụtara ụgwọ ha, ọdịdị na nha nke ụmụ irighiri ihe, enwere ike kewapụ protein dị iche iche site na electrophoresis. Serum nwere protein dị iche iche, ha niile nwere isi ihe isoelectric na pH7.5 ma ọ bụ obere. Mgbe etinyere serum na pH 8.6 buffer iji mee electrophoresis, a na-ebo protein niile nke serum ebubo na ọ ga-aga n'akụkụ dị mma na mpaghara eletrik. Ebe ọ bụ na protein dị iche iche nke serum nwere ụgwọ dị iche iche n'otu pH, ihe ndị dị na molekụla dị iche na nha, ya mere ọsọ ọsọ dị iche iche, na electrophoresis na-ekewa ha. A na-egosi isi ihe isoelectric na molecular arọ nke protein serum na tebụl dị n'okpuru:
| Protein | Isi ihe eletrik (PI) | Ibu molekụla |
| Albumin | 4.88 | 69 000 |
| α1-gloulin | 5.00 | 200 000 |
| α2-gloulin | 5.06 | 300 000 |
| β-gloulin | 5.12 | 9 000-150 000 |
| γ-gloulin | 6.85-7.50 | 156 000 ~ 300 000 |
Nnwale ahụ bụ ikewapụ protein dị iche iche na ọbara ọbara na cellulose acetate membrane (abbr. CAM) dị ka mgbasa ozi nkwado. CAM bụ ụdị ụfụfụeihe nkiri na ezigbo edo, na ọkpụrụkpụ bụ 0.1mm-1.5mm, nke nwere ụfọdụ mmiri absorption.
Akụrụngwa, ngwa na reagents maka CAM Electrophoresis
Nlereanya:ahụike ọbara mmadụ
Ngwa:ike ọkọnọ DYY-6C, electrophoresis tank DYCP-38C, elu sample loading WD-9404
Ndị reagents
1) cellulose acetate akpụkpọ anụ 7X9cm
2) PH 8.6 barbitol buffer solution (ionic ike 0.05-0.09, ọ bụ 0.06tid oge): were 1,84g Diethyl barbituric acid, wee were 1.03g Diethyl sodium pentobarbital, tinye ụfọdụ distilled mmiri ọkụ ka igbari, na-eme ka. ihe dị ka 1000 ml;
3) Unyi: Ponceau S 0.2g, Trichloroacetic 3g, tinye 100ml mmiri distilled;
4) TBS / T ma ọ bụ PBS / T: 45ml 95% ethanol, 5ml glacial acetic acid, tinye 50ml mmiri distilled;
5) Ngwọta nhicha: 70ml anhydrous ethanol, 30ml glacial acetic acid.
Ụzọ nnwaled
1) Kwadebe akpụkpọ ahụ: Na-emikpu akpụkpọ ahụ n'ime ihe ngwọta nke barbitol 30min-8h, wee wepụ ya, wepụ ihe ngwọta ahụ site na akwụkwọ na-adọrọ adọrọ.
2) Ihe nleba anya: Chọpụta akụkụ ahụ siri ike na akụkụ dị nro nke akpụkpọ ahụ, ma kaa akara na 1.5cm anya na njedebe elu n'akụkụ ahụ siri ike. Ibu ibu ihe nlere anya site na ngwa nledo kacha elu n'akụkụ nke siri ike. Mara: Ekwesịrị ịkwanye ihe nlele ahụ n'akụkụ akpụkpọ ahụ siri ike. Mgbe serum samples na-n'ụzọ zuru ezu penetrated n'ime akpụkpọ ahụ, tụgharịa akpụkpọ ahụ, na-etinye ike ike n'akụkụ (na samples) ihu ala na tank, na njedebe na sample-etinye na-adịghị mma electrode.
3) Electrophoresis: Gbanyụọ ọkụ ọkụ, 0.4～0.6m A / cm akpụkpọ ahụ, oge ịgba ọsọ bụ 30-45 min. Mgbe ịgbasa electrophoresis, gbanyụọ ike.
4) Unyi ma dị ọcha: Wepụ akpụkpọ ahụ na tank, na-emikpuit n'ime ihe ngwọta agba maka 5 min, wee kpochaa ya na ngwọta nhicha ugboro ugboro ugboro 3-4 ruo mgbe agba ndabere doro anya. Ekwesịrị igosi protein ndị na-edozi ahụ na eriri, ma na-enwekarị mpaghara ise, na-etolite n'elu njedebe na akara akara, Albumin, α1-gloulin, α2-gloulin, β-gloulin, γ-gloulin.
5) Nchekwa: tinye akọrọ elecrtophoresisbandna ngwọta nhicha maka 10-15 nkeji, wee wepụ ya ma tinye ya na iko dị ọcha, mgbe ọ nwụsịrị, ọ ga-aghọ ihe nkiri na-egbuke egbuke.band.
NnwaleNsonaazụ
Mmetụta nkewapụ nke ihe nlele serum dị mma, ọ nweghịkwa ihe ọ bụla na-eme ogologo oge. Ntughari nke nsonaazụ dịgasị iche n'ihi usoro nyocha na usoro nke onye nyocha, na nkwughachi dị elu.
Mmechi
Sistemụ nchọpụta electrophoresis nke ụlọ ọgwụ ngwa ngwa (Electrophoresis tankDYCP-38C,ịnye ọkụDYY-6C na elu sample loading WD-9404) nke anyị mepụtaraỤlọ ọrụ Beijing Liuyi Biotechnology Co., Ltdna-emezu ihe nnwale achọrọ,naNsonaazụ bụ reproducible, dị mfe na ngwa ngwa, adabarankuzinyocha.
Beijing LiuyiBiotechnology Co., Ltd ọkachamara n'ichepụta ngwaahịa electophoresis maka ụlọ ọrụ sayensị ndụ, nkenwere ihe karịrị afọ 50 akụkọ ihe mere eme na China na ụlọ ọrụ ahụ nwere ike ịnye ngwaahịa kwụsiri ike na nke dị elu gburugburu ụwa. Site na afọ nke mmepe, ọ bụ ihe kwesịrị ekwesị nke nhọrọ gị!
Anyị na-achọ ugbu a ndị mmekọ, ma OEM electrophoresis tank na nkesa na-anabata.
Maka ozi ndị ọzọ gbasara anyị, biko kpọtụrụ anyị site na email[email na-echebe]ma ọ bụ[email na-echebe].
Oge nzipu: Nov-14-2022