Agarosegel electrophoresis
Agarose gel electrophoresis bụ usoro gel electrophoresis nke a na-eji na biochemistry, biology biology, genetics, and clinical chemistry iji kewaa ọnụ ọgụgụ dị iche iche nke macromolecules dị ka DNA ma ọ bụRNA. Nke a na-enweta site na ịkwaga ụmụ irighiri ihe nucleic acid na-adịghị mma site na matriks agarose nwere.electrophoresis.
Njirimara nke agarose gel
Agarose gel bụ akụkụ atọ nke matriks kpụrụ nke helical agarose molecules na supercoiled bundles nke na-agbakọta n'ime akụkụ atọ akụkụ na ọwa na pores nke biomolecules nwere ike gafere. The 3-D Ọdịdị na-ejide ọnụ na hydrogen agbụ na ike ya mere a ga-akpaghasị. site na kpo oku laghachi na steeti mmiri mmiri. Okpomọkụ na-agbaze dị iche na gelling okpomọkụ, dabere na isi mmalite, agarose gel nwere okpomọkụ gelling nke 35.-42 Celsius C na okpomọkụ na-agbaze nke 85-95 Celsius C. Agaroses na-agbaze dị ala na nke dị ala nke emere site na mgbanwe kemịkalụ dịkwa.
Uru na ọghọm dị na agarose gel electrophoresis
Isi uru nke agarose gel electrophoresis technique bụ na enwere ike ịhazi ya ngwa ngwa na DNA molecule nke a na-eji dị ka ihe nlele nwekwara ike nwetaghachi ya n'enweghị mmerụ ọ bụla na njedebe nke usoro ahụ. N'ihi na agarose gel adịghị't nwere sulfate na otu carboxyl, ọ gaghị eme ka electroosmosis nke agar. Site na nhazi nke otu na nnukwu pore size, agarose gel dị mma makana-ekewa nnukwu mkpụrụ ndụ DNA, dịka enzyme mgbagwoju, nucleic acid na nje. Agarose gel adịghị't banye ìhè UV, ya mere enwere ike ime ya ozugbo ma ọ bụ mgbe gelling bụrụ ihe nkiri, ọ na-agbanwekwa ya nke ọma.
Enwekwara ụfọdụ ọghọm dị na teknụzụ agarose gel electrophoresis. Agarose gel na-agbaji ngwa ngwa, ya mere njupụta ahụ enweghị ike ịdị ala, ọ ga-erikwu agarose.Gel nke agarose ọ na-esiri ike ịzọpụta n'ihi na ọ dị mfe nje bacteria merụọ ya, ma ekwesịrị ịkwadebe ya tupu ejiri ya. N'iji tụnyere PAGE electrophoresis Usoro, arụmọrụ nke molekụla sieve dị obere, na mpaghara electrophoresis dị obere.
Kehoraizinelectrophoresisusoro maka agarose gel
Ewezuga agarose na ihe nchekwa, nnwale agarose gel electrophoresis chọrọ sistemu electrophoresis kwụ ọtọ na obere-voltaji electrophoresis ike ọkọnọ.Beijing Liuyi Biotechnology nwere ike na-enye kehoraizin electrophoresis usoro na ike ọkọnọ electrophoresis, dị ka ihe nlereanyaDYCP-31BN, DYCP-31CN makaSistemụ electrophoresis kwụ ọtọ naMMỤỌ-6C maka ike ọkọnọ.
Dabere n'ụdị gel dị iche ichechọrọ site na Agrose gel electrophoresis nnwale, dị iche iche nha nke kehoraizin electrophoresis usoro nwere ike inye site Beijing Liuyi Biotechnology. Ụdị usoro DYCP-31,sdị kaDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32B, naDYCP-32Cnwere nha dị iche iche nke jel tray, site na nkedo obere gel na oke gel, ha nwere ike izute nnwale gị.chọrọ.
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Oge nzipu: Eprel-16-2022