N'ebe a, anyị ga-akọwa otu esi eme agarose gel electrophoresis site n'aka onye nyocha anyị na ụlọ nyocha Liuyi Biotech.
Tupu nnwale ahụ, anyị kwesịrị ịlele ngwa, reagents, na ihe nnwale ndị ọzọ na ngwaọrụ anyị chọrọ.
Nkwadebe nke ngwa nnwale na ihe
Ngwa maka agarose gel electrophoresis
Igwe ikuku electrophoresis kwụ ọtọ (tank / ụlọ), etiti na ala voltaji electrophoresis ike ọkọnọ, ihe oyiyi gel & usoro nyocha.
Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) na-enye ụdị dị iche iche nke cell electrophoresis kwụ ọtọ (tank / ụlọ) na ike ọkọnọ yana usoro akwụkwọ gel. The nlereanya DYCP-31 usoro ngwaahịa bụ maka electrophoresis, na DYY usoro ngwaahịa bụ electrophoresis ike ọkọnọ. Ngwaahịa usoro WD-9413 bụ usoro akwụkwọ gel. Site na obere gel size 60 × 60mm ka oke gel 250 × 250mm, anyị nwere ike izute gị dị iche iche chọrọ maka gel size. Ihe nlereanyaDYCP-32Cnwere ike ime ka jel ruo nha 250 × 250mm. NkeMMỤỌ-6Cbụ ike anyị. Ọ na-akwado mmepụta nke 400V, 400mA, 240W, nke bụ ngwaahịa anyị na-emekarị nke ndị ahịa anyị na-eji. WD-9413BA na-eji Gel Image & Analysis System maka nyocha na nyocha maka gel, ihe nkiri, na blots mgbe nnwale electrophoresis gasịrị. Ọ bụ ngwaọrụ bụ isi nke nwere isi iyi ọkụ ultraviolet maka ikiri na ịse foto gels ndị nwere ihe eji esiji fluorescent dị ka ethidium bromide, yana isi iyi na-acha ọcha maka ịhụ anya na ịse foto gels ndị nwere agba dị ka coomassie na-acha anụnụ anụnụ.
Ndị na-ahụ maka agarose gel electrophoresis
1.The ọkara: agarose gel
2.Ihe nchekwa: TAE (Tris-acetate, EDTA, glacial acetic acid) na TBE (Tris-borate, EDTA, boric acid).
3.The loading buffer: 6 × DNA Loading Buffer (Pụrụ iche maka DNA sample: EDAT, glycerine, Xylene cyanol na bromophenol blue)
Ihe agbaji
Ihe eji esiji fluorescent dị ka EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
NkeIhe eji eme ihe
Ntuziaka pipette sterilization (10μL), pipette ndụmọdụ (200μL), pipette ndụmọdụ (1000μL), 200μL \ 500μL \ 1.5ml centrifuge tube.
Ihe nrịbama DNA
A na-akwado ya dabere na arọ molekụla a ga-anwale.
Gel na-agba ọsọ ma na-ekiri
Nke mbụ, anyị kwesịrị ịkwadebe gel agarose. Ntinye nke agarose na gel ga-adabere na nha nke iberibe DNA ga-ekewa, na ọtụtụ gels sitere na 0.5%-2%. Na-ewere anyịDYCP-31DNdịka ọmụmaatụ, Ị nwere ike ịkwanye obere gel, obosara gel , ogologo gel na square gel dị ka ihe achọrọ nke nnwale ahụ. Họrọ tray gel ị chọrọ, wee tinye mbo ahụ, wee wụsa gel agarose na-ekpo ọkụ n'ime ihe nkedo gel.
Mgbe ahụ, mgbe gel na-esiwanye ike, wepụ mbo ahụ nke ọma na nke dị nro, ma tinyezie tray gel n'ime tankị nchekwa. Wunye ngwọta nchekwa n'ime tankị nchekwa ma mee ka gel niile gbanye n'ime ihe nchekwa ahụ. Tinye ihe atụ ndị ahụ n'ime olulu mmiri na pipette ọkọlọtọ. JikọọDYCP-31DNna electrophoresis ike ọkọnọ n'ụzọ ziri ezi, ma tọọ paramita na-agba ọsọ gel.
Jiri anya nke uche hụ iberibe DNA gị na transilluminator UV.
AMgbe ị na-agba ọsọ gel, ị nwere ike iji ihe oyiyi gel anyị & usoro nyocha usoroWD-9413Biji lelee, nyochaa na see foto maka gel. Liuyi Biotech na-enyekwa UV transilluminator (UV Analyzer) ịhụ gel. Anyị nwere ụdị igbe ojii UV trihe atụ nke ansilluminator (UV Analyzer).WD-9403A, 9403C, WD-9403F, obere UV transilluminator (UV Analyzer) nlereanyaWD-9403Bna aka UV transilluminator (UV Analyzer)WD-9403En'ihi na ị na-ahọrọ.
Ihe onyonyo nke gel post electrophoresis
ika Liuyi nwere ihe karịrị afọ 50 nke akụkọ ihe mere eme na China na ụlọ ọrụ ahụ nwere ike ịnye ngwaahịa kwụsiri ike na nke dị elu gburugburu ụwa. Site na afọ nke mmepe, ọ bụ ihe kwesịrị ekwesị nke nhọrọ gị!
Maka ozi ndị ọzọ gbasara anyị, biko kpọtụrụ anyị site na email[email na-echebe], [email na-echebe].
Oge nzipu: Eprel-29-2022