Gel anaghị edozi nke ọma
Okwu: The gel nwere ụkpụrụ ma ọ bụ na-ezighị ezi, karịsịa na elu-etinye uche gels n'oge oyi oyi oyi, ebe ala nke kewara gel-egosi wavy.
Ngwọta: Mee ka ọnụọgụ polymerizing dị elu (TEMED na ammonium persulfate) iji mee ka usoro ntọala dị ngwa. Gbaa mbọ hụ na efere iko ndị ahụ dị ọcha iji gbochie ihe fọdụrụ na gels gara aga.
Gel dị nro
Okwu: Gel na-etinye uche dị ala (a na-eji maka protein dị arọ nke molekụla) na-agbaji n'oge njikwa.
Ngwọta: Jikwaa gel nke ọma n'oge usoro ahụ niile. N'ọnọdụ dị jụụ, na-abawanye ntakịrị ihe ndị na-emepụta polymerizing.
Gel anaghị edozi
Ngwọta: Na obere okpomọkụ, mee ka ọnụọgụ polymerizing dịkwuo elu. Gbaa mbọ hụ na ammonium persulfate adịla njikere. Ọ bụrụ na nsogbu ahụ na-aga n'ihu, doziegharịa ihe ngwọta nchekwa.
Ọtụtụ Streaks na Smears na Gel Mgbe Electrophoresis gasịrị
Ngwọta: Gbaa mbọ hụ na ngwọta gel dị ọcha yana agwakọta nke ọma. Jikwaa ojiji nke ihe nchekwa electrophoresis ma ọ bụ zere iji ya eme ihe kpamkpam. Ọ bụrụ na ị na-azụta ego na nchekwa, jiri nchekwa ọhụrụ n'ime ime ụlọ wee jirikwa ihe nchekwa ọzọ n'ọnụ ụlọ dị n'èzí.
Oge nhazi gel na-ezighi ezi
Okwu: Gel arụrụ n'ụlọ na-etinyekarị n'ime nkeji 30 ruo otu elekere, ebe ngwa azụmahịa na-etinye n'ime nkeji 10-20. Ọ bụrụ na ntọala adịchaghị ngwa ngwa, usoro onyunyo nke onye na-emepụta polymer nwere ike ọ gaghị ezu oke. Ọ bụrụ na ntọala dị oke ngwa ngwa, usoro onunu ogwu nwere ike ịdị elu, na-eme ka gel ahụ sie ike ma nwee ike ịgbawa, yana ịbawanye ohere nke ikpo ọkụ n'oge electrophoresis.
Ngwọta: Gbanwee ọnụọgụ nke ndị ọrụ polymerizing dabere na ọnọdụ ndị dị adị. Oge nhazi gel na-emetụta nke ọma site na okpomọkụ.
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Oge nzipu: Jul-29-2024