Okwu mmalite
Gel electrophoresis bụ usoro dị mkpa na usoro ihe ọmụmụ ihe ndụ, nke a na-ejikarị eme ihe maka nkewa nke protein, acid nucleic, na macromolecules ndị ọzọ. Ijikwa olu nlele nke ọma, voltaji, na oge electrophoresis dị oke mkpa iji nweta nsonaazụ ziri ezi na enwere ike imepụtaghachi.Onye otu ụlọ nyocha anyị na-enyeomume kacha mma maka ijikwa parampat ndị a n'oge SDS-PAGE gel electrophoresis.
Ngwa ngwaahịa jel electrophoresis nke Liuyi Biotechnology
Ọmụmaatụ olu: Na-eme ka ọ kwụsie ike
Mgbe ị na-eme SDS-PAGE electrophoresis, olu nlele bụ isi ihe nwere ike imetụta mkpebi nke nsonaazụ gị. A na-atụkarị aro ka ibu 10µL nke ngụkọta protein kwa olulu mmiri. Iji hụ na ọ na-agbanwe agbanwe ma gbochie mgbasa nke nlele n'etiti olulu mmiri dị n'akụkụ, ọ dị mkpa ịkwanye nha nha nha nke 1x loading buffer na olulu mmiri ọ bụla tọgbọ chakoo. Akpachara anya a na-enyere aka igbochi mgbasa nke sample n'ime ụzọ ndị agbata obi, nke nwere ike ime ma ọ bụrụ na olulu mmiri ahụ tọgbọ chakoo.
Tupu ibunye ihe nlele gị, malite mgbe niile site n'itinye akara nrịbama molekụla n'otu olulu mmiri. Nke a na-enye ohere maka mfe njirimara nke protein nha mgbe electrophoresis.
Njikwa voltaji: ịhazi ọsọ na mkpebi
Voltaji etinyere n'oge electrophoresis na-emetụta ma ọsọ nke ihe nlele na-aga site na gel na mkpebi nke nkewa. Maka SDS-PAGE, ọ dị mma ịmalite na obere voltaji nke gburugburu 80V. Igwe ọkụ mbụ a dị ala na-enye ohere ka ihe nlele ahụ jiri nwayọọ nwayọọ na-akwaga, na-etinye uche ha na eriri dị nkọ ka ha na-abanye na gel nkewa.
Ozugbo samples abanyela n'ụzọ zuru ezu na gel nkewa, enwere ike ịbawanye voltaji ruo 120V. Nke a dị elu voltaji na-eme ka mbugharị ahụ dịkwuo elu, na-ahụ na ndị na-edozi ahụ na-ekewa nke ọma dịka ịdị arọ ha si dị. Ọ dị mkpa iji nyochaa ọganihu nke bromophenol acha anụnụ anụnụ n'ihu, nke na-egosi mmecha nke electrophoresis. Maka gels nwere ntinye nke 10-12%, 80-90 nkeji bụ nke zuru oke; Otú ọ dị, maka 15% gels, ị nwere ike ịgbatị oge ọsọ ahụ ntakịrị.
Nlekọta oge: Ịmara mgbe a ga-akwụsị
Oge bụ ihe ọzọ dị oke mkpa na gel electrophoresis. Na-agba ọsọ gel ruo ogologo oge ma ọ bụ dị mkpirikpi oge nwere ike iduga nkewa nke ọma. Mbugharị nke bromophenol acha anụnụ anụnụ bụ ihe ngosi bara uru: mgbe ọ ruru ala nke gel, ọ na-abụkarị oge ịkwụsị ọsọ ahụ. Maka gels ọkọlọtọ, dị ka 10-12%, oge electrophoresis nke ihe dị ka nkeji 80-90 bụ nke zuru oke. Maka gels pasent dị elu, dị ka 15%, a ga-agbatị oge ịgba ọsọ iji hụ na nkewa zuru oke nke protein.
Njikwa ihe nkpuchi: Ejigharị na akwadokwa nchekwa
Enwere ike ijikwa ihe nchekwa Electrophoresis ugboro 1-2, dabere na ọnọdụ ụlọ nyocha gị akọwapụtara. Agbanyeghị, maka nsonaazụ kacha mma, a na-atụ aro ka ịkwadebe ihe nchekwa 10x ọhụrụ wee tụgharịa ya tupu ejiri ya. Nke a na-eme ka ihe nchekwa ahụ na-edobe ịdị irè ya, na-eduga na nsonaazụ electrophoresis ndị a pụrụ ịdabere na ya.
Ngwa ngwaahịa jel electrophoresis nke Liuyi Biotechnology
Site na iji nlezianya na-achịkwa olu nlele, voltaji, na oge electrophoresis, ị nwere ike imeziwanye izi ezi na nrụpụta nke nsonaazụ electrophoresis gel gị. Itinye usoro kachasị mma ndị a n'ọrụ ụlọ nyocha gị ga-enyere gị aka imeta ụgbụ dị iche iche na nke dị iche iche, na-eduga na data ka mma maka nyocha ala.
Ọ bụrụ na ị nwere ụzọ dị mma karị iji bulie nnwale electrophoresis gel, nabata ka gị na anyị kparịta ụka!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.
Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị. Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.
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Oge nzipu: Ọgọst-20-2024