Polyacrylamide gel Electrophoresis

A na-ejikarị polyacrylamide eme ihe na ngwa ihe ndị dị ndụ dị ka ihe ngwọta maka electrophoresis nke protein na nucleic acid na usoro a maara dị ka PAGE. Ọ bụ ụdị usoro electrophoresis mpaghara site na gel synthetics nke a na-akpọ polyacrylamide dị ka onye na-akwado ya. Ọ bụ S.Raymond na L.Weintraub wuru ya na 1959, wee kwalite ma mepụta ya site n'aka L.Ornstein na BJ Davis. A na-eji usoro a eme ihe n'ọtụtụ ebe ka ha nwekwuru nkọwa na mgbanwe na tiori na usoro nnwale na 1964.

Tupu itinye polyacrylamide gel electrophoresis, ndị mmadụ na-ejikarị akwụkwọ electrophoresis maka mpaghara EP. Ma akwụkwọ ahụ na-arụ ọrụ dị ka onye na-ajụ ase, dị nnọọ nwere ọrụ nke mgbochi convection, ọ dịghị ihe ọ bụla ọzọ mmetụta dị mma. Ọ bụ ezie na gel polyacrylamide abụghị naanị nwere ọrụ mgbochi convection kamakwa ọ nwere ike itinye aka na nkewa ahụ na-aga n'ihu. N'ihi na polyacrylamide gel bụ ụdị nhazi netwọk, nke bụ polymerization na njikọ njikọ nke acrylamide (Acr) na N, N-methylenebis (acrylamide). A na-akpọ Acrylamide monomer, ebe N, N-methylenebis ka a na-akpọ comonomer ma ọ bụ crosslinker. Ịmepụta gel bụ usoro nke chemical polymerization. Enwere ike ịchịkwa oghere nke gel, yabụ enwere ike ịme gel na ogo njikọ dị iche iche. Ọ bụrụ na nha pore na-abịaru nso na nkezi radius nke molecule sample, nguzogide nke molekul na-agafe na gel pore ga-enwe mmekọrịta chiri anya na nha na ọdịdị nke molekul n'oge electrophoresis. Ya mere ọ na-enye ihe nkewa nwere ike ịgbanwe maka ikewapụta ihe ndị ahụ nwere ụgwọ netwọlụ yiri nke ahụ.

Enwere ụzọ abụọ n'ozuzu eji eme polyacrylamide gel electrophoresis, otu bụ diski electrophoresis, nke ọzọ bụ slab electrophoresis. A na-ahazi slab electrophoresis nke ọma maka ikewa protein na DNA, na n'ozuzu, e nwere ụdị abụọ nke electrohporesis slab, nke bụ tank electrophoresis kwụ ọtọ na tank electrophoresis kwụ ọtọ. Maka protein ahụ, ndị mmadụ na-eji tankị electrophoresis kwụ ọtọ maka IFF na immunoelectrophoresis, ma ọ bụghị ya, ndị mmadụ na-eji tankị electrophoresis kwụ ọtọ maka protein.

Beijing Liuyi Biotechnology nwere ụdị dị iche iche nke slab electrophoresis tank maka PAGE, belụsọ maka nyocha na njirimara protin n'omume site na polyacrylamide gel electrophoresis, enwere ike iji ya tụọ nha molekụla nke ihe nlele, na-eme ka ihe dị ọcha na ịkwadebe ihe nlele.

Were ihe nlereanyaDYCZ-23Adị ka ihe atụ, nke bụ ahụkarị vetikal electrophoresis tank maka Lab. Jiri efere iko abụọ mepụta ọnụ ụlọ gel iji mee gel, wee kụọ efere iko ahụ nke ọma iji gbochie mgbapu gel. Ọkpụrụkpụ nke gel na-adabere na ọkpụrụkpụ nke spacer. Nọmalị, ọkpụrụkpụ bụ 1.5mm n'ihi na mma ntọhapụ okpomọkụ, naDYCZ-23Ana-enyekwa oghere 1.0mm ọkpụrụkpụ maka nkedo 1.0mm gel. Ewezuga tankị electrophoresis, iji na-agba ọsọ electrophoresis, a na-achọkwa ọkụ ọkụ. Beijing Liuyi Biotechnology na-enye ụdị dị iche icheelectrophoresis ike ọkọnọ. Site na voltaji dị elu na voltaji dị ala, ị na-ahọrọ ihe nlereanya dịka ngwa ahụ si dị.

The Beijing Liuyi ika nwere ihe karịrị 50-afọ akụkọ ihe mere eme na China na ụlọ ọrụ nwere ike na-enye kwụsiri ike na elu-edu ngwaahịa gburugburu ụwa. Site na afọ nke mmepe, ọ bụ ihe kwesịrị ekwesị nke nhọrọ gị!

Anyị na-achọ ugbu a ndị mmekọ, ma OEM electrophoresis tank na nkesa na-anabata.

Maka ozi ndị ọzọ gbasara anyị, biko kpọtụrụ anyị site na email[email na-echebe]ma ọ bụ[email na-echebe].