Polymerase Chain Reaction (PCR) na Gel Electrophoresis: Usoro dị mkpa na ihe ọmụmụ ihe omimi.

N'ihe gbasara usoro ihe ọmụmụ ihe na-agbanwe agbanwe, Polymerase Chain Reaction (PCR) na Gel Electrophoresis apụtala dị ka usoro nkuku nke na-eme ka ọmụmụ ihe na nhazi nke DNA dị mfe. Usoro ndị a abụghị naanị ihe dị mkpa na nyocha mana ha nwekwara ngwa zuru ebe niile na nyocha nchọpụta, sayensị nyocha na biotechnology.

Foto sitere na weebụsaịtị biology4alevel

PCR bụ usoro mgbanwe nke Kary Mullis mepụtara na 1983, nke na-enye ndị ọkà mmụta sayensị ohere ịbawanye otu akụkụ DNA nke ọma. Usoro a na-amalite site na denaturation nke DNA, ebe DNA nke nwere eriri abụọ na-ekpo ọkụ na gburugburu 94 Celsius, na-eme ka ọ kewaa n'ime eriri abụọ. Nke a na-esochi annealing, ebe primers-obere usoro nke nucleotides-jide na usoro mmekọ na DNA nke nwere otu eriri na obere okpomọkụ (na-emekarị gburugburu 55 ° C). N'ikpeazụ, usoro mgbatị ahụ na-eme na 72 Celsius C, ebe enzyme DNA polymerase na-ejikọta eriri DNA ọhụrụ site na ịgbakwunye nucleotides na primers. A na-emegharị okirikiri a ugboro 20-40, na-eduga n'ọtụtụ nde nde nke usoro DNA ezubere iche.

Igwe PCR nke Beijing LIUYI

Ozugbo DNA kwalitere, Gel Electrophoresis na-arụ ọrụ iji kewapụ na nyochaa ngwaahịa PCR. Usoro a na-agụnye mbugharị nke iberibe DNA site na matriks gel agarose n'okpuru mmetụta nke ọkụ eletrik. A na-ebo mkpụrụ ndụ DNA ebubo na-adịghị mma n'ihi ọkpụkpụ azụ phosphate ha, ha na-akwaga na electrode dị mma. Gel na-arụ ọrụ dị ka sieve, na-ekwe ka obere iberibe DNA na-aga ngwa ngwa karịa ndị buru ibu. N'ihi ya, a na-ekewa iberibe DNA na-adabere na nha ha, na-enwe eriri dị iche iche a na-ahụ n'okpuru ìhè ultraviolet mgbe agbachara agba dị ka ethidium bromide.

Beijing LIUYINgwaahịa Gel Electrophoresis

Nchikota PCR na Gel Electrophoresis dị ike n'ọtụtụ ngwa. Na nyocha ahụike, a na-eji PCR chọpụta ọnụnọ nke ọrịa nje, mmụgharị mkpụrụ ndụ ihe nketa, ma ọ bụ usoro DNA akọwapụtara nke metụtara ọrịa. Gel Electrophoresis na-enye ohere maka ịhụ anya na nkwenye nke iberibe DNA ndị a gbasiri ike. Na sayensị nyocha, usoro ndị a dị oke mkpa maka akara mkpịsị aka DNA, ebe ha na-enyere aka ijikọ ihe nlele DNA sitere na ọnọdụ mpụ na nke ndị a na-enyo enyo.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.

Beijing LIUYINgwaahịa Gel Electrophoresis

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