Na-akwadebe Gel Agarose maka Electrophoresis

Nkwadebe nke Agarose Gel maka Electrophoresis

Mara: Yiri gloves enwere ike iwepu mgbe niile!

Ntuziaka nzọụkwụ site na nzọụkwụ

Na-atụ ntụ ntụ Agarose:use na-atụle akwụkwọ na nguzozi eletrọnịkị iji tụọ 0.3g nke ntụ ntụ agarose (dabere na sistemụ 30ml).

Na-akwado ihe nchekwa TBST:prụkwaa 30ml nke 1x TBST nkwụnye n'ime 100ml Erlenmeyer flask.

Na-agbaze ntụ ntụ Agarose:pntụ ntụ agarose anyị n'ime ebe nchekwa TBST wee maa jijiji nke ọma.Tinye ha n'imena ngwa ndakwa nri na okpomọkụ (nke a na-emekarị maka 50 sekọnd, kpuchie na aluminom foil) ruo mgbe agbazere kpamkpam.

Ịjụ na Ịtinye Nuclease:use gloves iji wepụ ngwakọta na ngwa ndakwa nri ma hapụ ya ka ọ dị jụụ na mmiri oyi ruo mgbe ọ dị ọkụ (ihe dịka 60 ° C). Tinye 2µl nke nuclease (Eb nnọchi) ka ị na-ama jijiji ka ị gwakọta nke ọma.

Ịkwadebe Akpụkpọ Gel:

• Cdabere ma kpoo yajel tray na gel nkedo ngwaọrụnke electrophoresis tank.
•  Pakwa geltrayn'ime tank nke dị n'ime ma tinye mbo na ọnọdụ a kapịrị ọnụ.
• Gwakọta ihe ngwọta agarose, dị jụụ ruo ihe dịka 65 Celsius C, ma jiri nlezianya wụsa ya na yajel trayn'imengwaọrụ nkedo gel, na-agbasa ya nwayọọ nwayọọ ruo mgbe ọ na-etolite ọbụna gel Layer na efere iko.
• Kwe ka ọ nọdụ na ụlọ okpomọkụ ruo mgbe gel na-agbaze kpamkpam.
• Jiri nwayọ wepu mbo ahụ kwụ ọtọ wee wepụ teepu ahụ.
• Tinye geltrayn'ime tank nke electrophoresis.

Ihe dị mkpa: Gbaa mbọ hụ na ọ nweghị afụ ikuku na mpaghara nha nha. Elu nke mmiri mmiri kwesịrị ịdị nro na-enweghị ripples.

Na-agba ọsọ Gel

Na-ebu Gel

Mgbe gel siri ike, tinye ya n'ime tank electrophoresis ma wụsa ihe nchekwa electrophoresis ruo mgbe mmiri na-emikpu.

Na-akwado nlele

• Were ihe nrịbama na ihe nkwụnye n'ime ngwa nju oyi.
• Tinye 6µl nke ihe nkwụnye ihe na nlele ma gwakọta nke ọma.
• N'iji micropipette, jiri nwayọọ na-ebu ihe nlele ndị ahụ n'ime nnukwu olulu mmiri nke gel (kpachara anya ka ị ghara ịkụnye gel ma ghara ịgbasa ụda dum ka ịzere ikuku ikuku).
• Tinye akara n'ime obere olulu mmiri ọ bụla (cheta ọnọdụ ya).

Malite Electrophoresis

• Na-ekpuchi tank nke electrophoresis ma malite electrophoresis ozugbo ị wụnye gel.
• Tọọ voltaji na 60-100V. Ihe nlele ahụ ga-esi na electrode na-adịghị mma (oji) gaa na electrode ziri ezi (red).
• Igwe ọkụ dị elu na-ebelata oke nkewa dị irè nke gel agarose.
• Kwụsị electrophoresis mgbe agba anụnụ anụnụ bromophenol ruru ihe dịka 1cm site na nsọtụ ala nke efere jel.

Na-ahụ nsonaazụ

Mgbe ị kewasịrị eriri ndị ahụ, kwụsị electrophoresis, wepụ gel ahụ, chọpụta ozugbo wee see ya foto.

Jiri sistemu onyonyo gel ka ịse foto ma lelee ụgbụ a (leba anya ma ọ bụrụ na enwere eriri maka akara ma ọ bụ ihe nlele).

Mgbe ị nwetachara map band gel gị, chọta akara ahụ. Dabere na ihe nrịbama, ị nwere ike ikpebi ndị agha ebumnuche!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ka arụpụtara ngwaahịa electrophoresis ihe karịrị afọ 50. Anyị na-ewetara sistemu electrophoresis kwụ ọtọ ebe a makaagarose gelelectrophoresis.HọrọSistemụ electrophoresis kwụ ọtọsitere na Beijing liuyi maka nnwale electrophoresis gel gị iji nyere gị aka nyocha na ụlọ ọrụ teknụzụ biotechnology.

Anyị na-enye ụdị dị iche iche nke tankị electrophoresis kwụ ọtọ maka nkedo na ịgba ọsọ obereagarosegels na nnukwuagarosejel

Ntuziaka nhọrọ maka ngalaba Gel Electrophoresis kwụ ọtọ

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.

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