Anyị ekekọrịtala ụfọdụ okwu ndị a na-ahụkarị gbasara ụdọ electrophoresis na mbụ, na anyị'Ọ ga-amasị m ịkọrọ ụfọdụ ihe na-adịghị mma nke polyacrylamide gel electrophoresis n'akụkụ nke ọzọ. Anyịchịkọta okwu ndị a maka ndị ahịa anyị' ntụaka iji chọpụta ihe kpatara ya na kanweta nsonaazụ ziri ezi ma melite ogo nkewa protein.
Gel anaghị eme ka ọ bụrụ polymer enwere ike
Ihe kpatara ya bụ:
(a) Ịdị ọcha monomer ezughi oke, na-achọ recrystallization.
(b) ikuku oxygen gbazere na ngwọta gel na-egbochi gel polymerization. Ekwesịrị iji mgbapụta sparging dị irè.
(c) Ammonium persulfate adịghị arụ ọrụ ma ọ bụ ezughi oke na oke. Kwadebe ma ọ bụ jiri ogbe ọzọ nke ammonium persulfate nwere itinye uche dị elu.
Tọ jel emeworị polymerized na-enweghị ịgbakwunye mmiri oyi akwa.
Ihe kpatara ya bụ:
(a) Mee ka ngwọta gel dị jụụ tupu ị gbakwunye ihe na-akpali akpali iji belata ọnụego polymerization.
(b) Belata ego TEMED ma ọ bụ ammonium persulfate eji.
(c) Mee ka ọrụ ahụ dị ngwa.
Gel na-esi na ụlọ electrophoresis pụta mgbe polymerization gasịrị
Nke a na-adịkarị mfe ime na gels dị ala. Otu ihe ngwọta bụ ka a kechie akpụkpọ ahụ dialysis n'akụkụ ala nke tube ma ọ bụ jiri isi polypropylene nwere oghere.
Achọpụtaghị ihe nlele mgbe electrophoresis gasịrị
Ihe kpatara ya bụ:
(a) Ego nlele ezughi oke. Tinye ego nlele.
(b) Njirimara ngwọta ncha ma ọ bụ itinye uche ma ọ bụ oge ntụpọ ezughi oke. Dochie ngwọta nchacha ma mee ka ntinye uche na oge nkwụsị.
(c) The sample sere n'elu mgbe loading. Mụbaa njupụta nke ngwọta nlele ma jiri nlezianya mee ihe.
(d) Nchịkọta gel nkewa dị oke elu, ihe nlele ahụ abanyeghịkwa na gel. Gbanwee mkpokọta gel nke ọma.
(e) Ntuba gel nkewa dị oke ala, a na-ewepụkwa ihe nlele ahụ site na gel nkewa. Gbanwee mkpokọta gel na ọnọdụ electrophoresis kacha mma.
(f) Ọ bụrụ na ihe nlele ahụ bụ RNA, ọ nwere ike ịnwe protein ndị na-emepụta nnukwu mgbagwoju anya nke na-egbochi oghere gel. Wepu protein nke ọma na nlele RNA.
(g) Ihe nlele ahụ nwere enzymes nke na-eme ka ihe nlele ahụ na-emebi emebi, na ihe nlele ahụ na-emebi n'oge electrophoresis. Mee ka nlele ahụ dị ọcha nke ọma.
Beijing Liuyi Biotechnology Company Ltd na-arụpụta ọtụtụ ngwaahịa electrophoresis nke nwere ike inye aka dozie nsogbu ndị anyị nwere ike izute na nnwale electrophoresis anyị. E hiwere ụlọ ọrụ ahụ na 1970. Ọ bụ mba nwere ma mepụta igwe ịgbado ọkụ eletrik na mita eruba ụlọ ọrụ n'oge ahụ. Kemgbe 1979, Beijing Liuyi Biotechnology Company Ltd malitere imepụta ngwaahịa electrophoresis. Ugbu a ụlọ ọrụis otu n'ime ndị isionye na-emepụta akụrụngwa ụlọ nyocha na ngwa sayensị dabere na Beijing, China. Ya ụghalaahịa"LIUYI” ama ama na China na mpaghara a.
Ngwaahịa nke ụlọ ọrụ ahụ gụnyere ọtụtụ ngwa ụlọ nyocha, gụnyere kehoraizin nucleic acid electrophoresis tank, vetikal protein electrophoresis tank / unit, ojii-igbeụdị Onye nyocha UV,Gel Document Tracking Imaging Analyzer, na ọkụ ọkụ electrophoresis. A na-eji ngwaahịa ndị a eme ihe n'ọtụtụ ebe na ụlọ ọrụ nyocha, mahadum, na ụlọ ọrụ dị iche iche, dị ka ọgwụ, nkà na ụzụ, na nlekota gburugburu ebe obibi.The ụlọ ọrụ bụ ISO9001 & ISO13485 gbaara ụlọ ọrụ na nwere CE asambodo.
Enwereụdị dị iche ichevetikalelectrophoresis tank makaelectrophoresis proteinmaka nyocha na njirimara nke ihe atụ protein site na polyacrylamide gel electrophoresis,nanakwa maka ịlele sample 'molecular arọ, ọcha samples na-akwadebe samples.A na-anabata ngwaahịa ndị a niileahịa ụlọ na esenidụt.
The electrophoresis ike ọkọnọ bụ ihe dị mkpa akụrụngwa nkeusoro nke electrophoresis, na-enye ebe ọkụ eletrik kwụ ọtọ na nke ziri ezi iji mee ka usoro nkewa ahụ dị.Itna-ebunye voltaji na-adịgide adịgide ma ọ bụ ugbu a na sistemụ electrophoresis, dabere na ụkpụrụ nnwale akọwapụtara. Ọ na-enye ohere ka onye ọrụ gbanwee voltaji ma ọ bụ mmepụta ugbu a, yana ihe ndị ọzọ dị ka oge na okpomọkụ, iji kwalite ọnọdụ nkewa maka otu nnwale.
Fma ọ bụ na-ekiri gel, ị nwere ike họrọ UV Transilluminator WD-9403 usoro nke Beijing Liuyi Biotechnology rụpụtara.A UV transilluminator bụ ngwa ụlọ nyocha eji elele anya yana nyocha DNA, RNA na protin. Ọ na-arụ ọrụ site na iji ọkụ UV na-amụba ihe atụ ndị ahụ, nke na-eme ka ihe nlele ahụ na-egbuke egbuke ma bụrụ ndị a na-ahụ anya. Enwere ọtụtụ ụdị nke transilluminator UVnke anyị nyere gị. WD-9403A bụ nke pụrụ iche maka ịhụ protein electrophoresis, na WD-9403F na-eji maka ịhụ DNA na protein electrophoresis.
Usoro ngwaahịa ndị a nwere ike ije ozi site na nkedo gel ruo na-elele gel dịka ihe nnwale gị siri dị.Mee ka anyị mara ihe ị chọrọ, ma OEM, ODM na ndị nkesa anabatara.We ga-agbalị ike anyị niile na-enye gị ngwaahịa na ọrụ anyị.
Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị. Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.
Oge nzipu: Mee-23-2023