Ị nwere nsogbu ọ bụla na ịkwadebe agarose gel?Ka iso naonye na-ahụ maka ụlọ nyocha anyị na-akwadebe gel.
Usoro nkwadebe nke agarose gel gụnyere usoro ndị a:
Na-atụ Agarose ntụ ntụ
Tulee ihe achọrọ nke ntụ ntụ agarose dịka ntinye uche achọrọ maka nnwale gị. Ọnụnọ nke agarose nkịtị sitere na 0.5% ruo 3%. A na-eji ntinye uche dị elu maka ikewapụta ụmụ irighiri ihe DNA dị nta, ebe mkpokọta dị ala bụ maka ụmụ irighiri ihe buru ibu.
Na-akwado Ngwọta nchekwa
Tinye ntụ ntụ agarose na ebe nchekwa electrophoresis kwesịrị ekwesị, dị ka 1 × TAE ma ọ bụ 1 × TBE. Olu ihe nchekwa kwesịrị dakọtara na olu gel achọrọ maka nnwale gị.
Na-agbaze Agarose
Kpoo ngwakọta agarose na ihe nchekwa ruo mgbe agarose gbazere kpamkpam. Enwere ike ime nke a site na iji microwave ma ọ bụ efere ọkụ. Gwakọta ihe ngwọta ahụ n'otu oge ka ọ ghara isi ya. Ihe ngwọta agarose kwesịrị ịpụta ìhè n'enweghị ihe ọ bụla a na-ahụ anya.
Ngwọta Agarose dị jụụ
Kwe ka ihe ngwọta agarose dị ọkụ mee ka ọ dị jụụ ruo ihe dịka 50-60 Celsius. Gwakọta ihe ngwọta n'oge usoro jụrụ oyi iji gbochie ike siri ike.
Na-agbakwunye Unyi Nucleic Acid (Nhọrọ)
Ọ bụrụ na ịchọrọ iji anya nke uche hụ DNA ma ọ bụ RNA na gel, ị nwere ike itinye ntụpọ nucleic acid, dị ka GelRed ma ọ bụ ethidium bromide, n'oge a. Mgbe ị na-ejizi ntụpọ ndị a, yiri uwe aka ma kpachapụ anya, n'ihi na ha nwere ike ịdị na-egbu egbu.
Ịmepụta gel
Wunye ngwọta agarose jụrụ oyi n'ime ihe gel electrophoresis a kwadebere. Fanye mbo iji mepụta olulu mmiri ihe atụ, na-ahụ na mbo ahụ dị nchebe na a na-ekesa ngwọta ya n'otu n'otu n'ụdị ahụ.
Ngwakọta jel
Kwe ka gel sikwuo ike na ụlọ okpomọkụ, nke na-ewekarị 20-30 nkeji dabere na ntinye na ọkpụrụkpụ nke gel.
Rna-ebugharị Comb
Ozugbo gel ahụ gbasiri ike, jiri nlezianya wepụ mbo ahụ iji gosi olulu mmiri nlele. Tinye gel ya na ebu ahụ n'ime ụlọ electrophoresis ma kpuchie ya na ọnụọgụ electrophoresis kwesịrị ekwesị, na-ahụ na gel na-ejupụta n'ime ya.
Na-akwado maka Electrophoresis
Mgbe gel dị njikere, tinye ihe nlele gị n'ime olulu mmiri, ma gaa n'ihu na nnwale electrophoresis.
Ọ bụrụ na ị nwere nsogbu ọ bụla na nkwadebe gel, nweere onwe gị ịkpọtụrụ anyị. Anyị nwere ndị ọkachamara ụlọ nyocha dị ka gị na gị kparịta ụka.
Anyị nwere obi ụtọ ịkọrọ ụfọdụ ozi ọma: DYCP-31DN anyị na-ewu ewu tank electrophoresis na-akwalite ugbu a., kpọtụrụ anyị maka ozi ndị ọzọ ugbu a!
DYCP-31DN kehoraizin electrophoresis tank
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.
Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị. Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.
Biko lelee koodu QR ka ịgbakwunye na Whatsapp ma ọ bụ WeChat.
Oge nzipu: Ọktoba 15-2024