Kedu ihe bụ usoro ịgba ígwè ọkụ na PCR?

Polymerase Chain Reaction (PCR) bụ usoro ihe ọmụmụ ihe omimi nke a na-eji na-amụba iberibe DNA a kapịrị ọnụ. Enwere ike iwere ya dị ka ụdị pụrụ iche nke ntugharị DNA na-abụghị nke ihe dị ndụ. Akụkụ bụ isi nke PCR bụ ikike ya ịbawanye ọnụọgụ DNA nke ukwuu.

Nchịkọta nke okirikiri mmeghachi omume nrịanrịa polymerase

Foto sitere na Bioninja

Kedu ihe bụ usoro ịgba ígwè ọkụ na PCR?

PCR nwere usoro atọ bụ isi:

1. Denaturation nke DNA Template: DNA template na-ekpo ọkụ na gburugburu 93 Celsius maka oge ụfọdụ, na-eme ka DNA nke nwere eriri abụọ na-ekewa n'ime otu strands, na-akwadebe ya maka njikọ primer na usoro mmeghachi omume na-esote.

2. Mwepu nke DNA Template na Primers: Mgbe denaturation gasịrị, a na-ebelata okpomọkụ ruo ihe dịka 55 Celsius, na-enye ohere ka ndị na-emepụta ihe na-ejikọta na usoro mmeju na DNA nke nwere otu eriri.

3. Mgbatị nke Primers: Na 72 ° C, DNA template-primer complex na-enweta mgbatị site n'enyemaka nke DNA polymerase (dị ka Taq DNA polymerase), na-eji dNTP dị ka ihe ntinye iji mepụta eriri ọhụrụ na-akwado dabere na DNA template.

Igwe PCR nke Beijing LIUYI

Site n'ịmeghachi usoro denaturation, annealing, na ndọtị, a na-emepụta ọtụtụ "ụkpụrụ mmegharị nke ọkara-conservative", nke nwere ike bụrụ ndebiri maka okirikiri ndị na-esote. Ọkpụkpụ nke ọ bụla na-ewe ihe dị ka nkeji 2 ruo 4, na-enye ohere ka mkpụrụ ndụ ihe nketa ebu n'obi gbasaa ọtụtụ nde ugboro n'ime awa 2 ruo 3.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) nwere specialized na n'ichepụta electrophoresis ngwá n'ihi na ihe karịrị 50 afọ na anyị onwe anyị ọkachamara oru otu na R&D center. Anyị nwere ahịrị mmepụta a pụrụ ịdabere na ya zuru oke site na imewe ruo nyocha, na ụlọ nkwakọba ihe, yana nkwado ahịa. Anyị isi ngwaahịa bụ Electrophoresis Cell (tank / ụlọ), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System wdg Anyị na-enye ụlọ nyocha ngwá ọrụ dị ka PCR ngwá, vortex igwekota na centrifuge maka laabu.

Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị. Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.

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