PCR Thermal Cycler WD-9402D
Nkọwapụta
| Nlereanya | WD-9402D |
| Ike | 96 × 0.2 ml |
| Tube | 0.2ml tube, 8 ibe, Ọkara skirt96 efere olulu mmiri, Enweghị uwe mwụda 96 olulu mmiri |
| Olu mmeghachi omume | 5-100ul |
| Oke okpomọkụ | 0-105 ℃ |
| MAX. Ọnụego mgbago | 5 ℃/s |
| Ịdị n'otu | ≤±0.2℃ |
| Izi ezi | ≤±0.1℃ |
| Ngosipụta mkpebi | 0.1 ℃ |
| Njikwa okpomọkụ | Mgbochi/Tube |
| Enwere ike idozi ọnụego Ramping | 0.01-5 ℃ |
| Okpomọkụ gradient. Oke | 30-105 ℃ |
| Ụdị gradient | Nkịtị gradient |
| Mgbasa gradient | 1-42 ℃ |
| Okpomọkụ mkpuchi ọkụ | 30-115 ℃ |
| Ọnụọgụ nke mmemme | 20000 + (USB FLASH) |
| Oke. Nọmba nke Nzọụkwụ | 40 |
| Oke. Nọmba nke okirikiri | 200 |
| Mmụba/Mbelata oge | 1 nkeji - 600 sekọnd |
| Mmụba/Mbelata okpomọkụ | 0.1-10.0 ℃ |
| Ọrụ kwụsịtụrụ | Ee |
| Nchekwa data akpaaka | Ee |
| Jide na 4 ℃ | Ruo mgbe ebighị ebi |
| Ọrụ mmetụ | Ee |
| Ogologo PCR ọrụ | Ee |
| Asụsụ | Bekee |
| Ngwa kọmputa | Ee |
| Ekwentị mkpanaaka APP | Ee |
| LCD | 10.1 inch, 1280×800 pel |
| Nkwukọrịta | USB2.0, WIFI |
| Akụkụ | 385mm × 270mm × 255mm (L×W×H) |
| Ibu | 10kg |
| Ịnye ọkụ | 100-240VAC, 50/60Hz, 600 W |
Nkọwa
Igwe igwe na-ekpo ọkụ na-arụ ọrụ site na kpo oku ugboro ugboro na ime ka ngwakọta mmeghachi omume nwere DNA ma ọ bụ RNA template, primers, na nucleotides. A na-achịkwa ịnya igwe okpomọkụ nke ọma iji nweta denaturation, mgbakasị ahụ na usoro ndọtị dị mkpa nke usoro PCR.
Dị ka ọ na-adịkarị, igwe na-ekpo ọkụ na-ekpo ọkụ nwere ngọngọ nwere ọtụtụ olulu mmiri ma ọ bụ tubes ebe a na-etinye ngwakọta mmeghachi omume, a na-achịkwa okpomọkụ dị na olulu mmiri ọ bụla n'onwe ya. A na-ekpo ọkụ ma mee ka mgbochi ahụ dị jụụ site na iji ihe Peltier ma ọ bụ usoro kpo oku na oyi ọzọ.
Imirikiti ndị na-agba ọkụ na-ekpo ọkụ nwere interface enyi na enyi nke na-enye onye ọrụ ohere ịhazi ma mezie usoro ịgba ígwè, dị ka okpomọkụ na-ekpo ọkụ, oge mgbatị, na ọnụ ọgụgụ nke okirikiri. Ha nwekwara ike ịnwe ihe ngosi iji nyochaa ọganihu nke mmeghachi omume ahụ, ụfọdụ ụdị nwere ike ịnye atụmatụ dị elu dị ka njikwa okpomọkụ gradient, nhazi ọtụtụ ngọngọ, na nlekota na njikwa anya.
Ngwa
genome cloning; Nkwadebe PCR asymmetric nke DNA nwere otu eriri maka usoro DNA; PCR na-agbanwe agbanwe maka mkpebi nke mpaghara DNA amaghị; ntụgharị ntụgharị PCR (RT-PCR). Maka ịchọpụta ọkwa mkpụrụ ndụ ihe nketa na mkpụrụ ndụ, yana ọnụọgụ nje RNA na cloning kpọmkwem nke cDNA nwere mkpụrụ ndụ ihe nketa akọwapụtara; mmụba ngwa ngwa nke cDNA agwụ; nchọpụta nke mkpụrụ ndụ ihe nketa; enwere ike itinye ya na nchọpụta nke ọrịa nje na nje virus; nchọpụta nke ọrịa mkpụrụ ndụ ihe nketa; nchọpụta nke etuto ahụ; enweghị ike iji nyocha ahụike dị ka ihe akaebe anụ ahụ nke forensic, na nyocha ụlọ ọgwụ ọgwụ.
Egosiputara
• Oke kpo oku na nju oyi, max. Ọnụego mgbago 8 ℃/s;
• Malitegharịa ekwentị na akpaghị aka mgbe ike dara. Mgbe ike weghachiri eweghachi ọ nwere ike ịga n'ihu na-eme mmemme emechabeghị;
• Otu-pịa ngwa ngwa incubation ọrụ nwere ike izute nnwale mkpa dị ka denaturation, enzyme ịkpụ / enzyme-njikọ na ELISA;
• Enwere ike ịtọ okpomọkụ mkpuchi ọkụ na ọnọdụ ọrụ mkpuchi ọkụ iji gboo mkpa nnwale dị iche iche;
• Na-eji ọnọdụ okpomọkụ-kpọmkwem ogologo ndụ Peltier modul;
• Modul aluminum anodized na nkwado injinia, nke na-ejigide arụmọrụ ọkụ ọkụ ngwa ngwa ma nwee nguzogide corrosion zuru oke;
• Ọnụọgụ okpomọkụ dị ngwa ngwa, yana oke ịrị elu nke 5 Celsius C / s, na-echekwa oge nnwale bara uru;
• Nrụgide nrụgide mmanya-ụdị mkpuchi okpomọkụ, nke nwere ike imechi ya nke ọma na otu nzọụkwụ ma nwee ike ime mgbanwe na elu tube dị iche iche;
• Nhazi ikuku nke ihu na azụ, na-ekwe ka a na-etinye igwe n'akụkụ n'akụkụ;
• Na-eji sistemụ arụmọrụ gam akporo, jikọtara ya na ihuenyo mmetụ aka 10.1-inch capacitive, yana interface igodo ụdị menu eserese, na-eme ka ọrụ dị oke mfe;
• Ihe ndebiri faịlụ mmemme ọkọlọtọ 11 arụnyere, nke nwere ike dezie faịlụ achọrọ ngwa ngwa;
• Ngosipụta oge nke ọganihu mmemme na oge fọdụrụnụ, na-akwado mmemme etiti nke ngwa PCR;
• Otu bọtịnụ ngwa ngwa incubation ọrụ, na-egbo mkpa nke nnwale dị ka denaturation, enzyme mgbaze / ligation, na ELISA;
• Enwere ike ịtọ okpomọkụ mkpuchi na-ekpo ọkụ na mkpuchi na-ekpo ọkụ na-arụ ọrụ iji gboo mkpa nnwale dị iche iche;
• Nchekwa ọkụ na-akpaghị aka, na-eme ihe na-akpaghị aka cycles mgbe emechara ike, na-eme ka ọrụ nchekwa na-arụ ọrụ n'oge usoro nkwalite;
• USB interface na-akwado PCR data nchekwa/iweghachi site na iji eriri USB ma nwee ike iji eriri USB jikwaa ngwa PCR;
• Na-akwado mmelite ngwanrọ site na USB na LAN;
• modul WIFI arụnyere n'ime ya, na-enye ohere ka kọmputa ma ọ bụ ekwentị mkpanaaka jikwaa ọtụtụ ngwa PCR n'otu oge site na njikọ netwọkụ;
• Na-akwado ọkwa email mgbe mmemme nnwale zuru.
FAQ
Ajụjụ: Gịnị bụ thermal cycler?
A: Igwe igwe na-ekpo ọkụ bụ ngwaọrụ ụlọ nyocha eji eme ka DNA ma ọ bụ RNA dị elu site na mmeghachi omume polymerase chain (PCR). Ọ na-arụ ọrụ site na ịgba ígwè site na mgbanwe mgbanwe okpomọkụ, na-ekwe ka usoro DNA dị iche iche gbasaa.
Ajụjụ: Gịnị bụ isi ihe nke a thermal cycler?
A: Isi ihe dị na cycler thermal gụnyere ihe mgbochi ọkụ, igwe ọkụ ọkụ, ihe mmetụta okpomọkụ, microprocessor, na ogwe njikwa.
Ajụjụ: Kedu ka igwe igwe thermal si arụ ọrụ?
A: Igwe igwe na-ekpo ọkụ na-arụ ọrụ site na ikpo ọkụ na ime ka ihe atụ DNA dị jụụ n'usoro usoro okpomọkụ. Usoro ịgba ígwè gụnyere denaturation, annealing, na ndọtị nkebi, nke ọ bụla nwere kpọmkwem okpomọkụ na ogologo oge. Usoro okirikiri ndị a na-enye ohere ka amụba usoro DNA dị iche iche site na mmeghachi omume polymerase chain (PCR).
Ajụjụ: Kedu ihe ndị dị mkpa ị ga-atụle mgbe ị na-ahọrọ cycler thermal? A: Ụfọdụ atụmatụ dị mkpa ị ga-atụle mgbe ị na-ahọrọ cycler thermal gụnyere ọnụ ọgụgụ nke olulu mmiri ma ọ bụ mmeghachi omume mmeghachi omume, oke okpomọkụ na ọsọ rampụ, izi ezi na ịdị n'otu nke njikwa okpomọkụ, na njirimara onye ọrụ na ike ngwanrọ.
Ajụjụ: Kedu ka ị ga-esi na-edobe cycler thermal?
A: Iji nọgide na-enwe cycler thermal, ọ dị mkpa ka ị na-ehicha ihe mgbochi kpo oku na tubes mmeghachi omume mgbe niile, lelee anya na-eyi na ihe ndị dị na ya, na calibrate ihe mmetụta okpomọkụ iji hụ na njikwa okpomọkụ ziri ezi na nke na-agbanwe agbanwe. Ọ dịkwa mkpa ịgbaso ntuziaka onye nrụpụta maka nhazi na nrụzi oge niile.
Ajụjụ: Kedu ụfọdụ usoro nchọpụta nsogbu a na-ahụkarị maka cycler thermal?
A: Ụfọdụ usoro nchọpụta nsogbu a na-ahụkarị maka igwe igwe ọkụ na-agụnye ịlele maka ihe ndị rụrụ arụ ma ọ bụ mebiri emebi, nyochaa ọnọdụ okpomọkụ na oge kwesịrị ekwesị, na ịnwale tubes mmeghachi omume ma ọ bụ efere maka mmetọ ma ọ bụ mmebi. Ọ dịkwa mkpa ịtu aka na ntuziaka onye nrụpụta maka usoro nchọpụta nsogbu na ngwọta.
