Ubi Gel Electrophoresis CHEF Mapper A1
Nkọwapụta
| Nlereanya | CHEF Mapper A1 |
| Voltaji gradient | 0.5V/cm ruo 9.6V/cm, gbakwunyere site na 0.1V/cm |
| Kachasị ugbu a | 0.5A |
| Voltaji kacha | 350V |
| Akụkụ Pulse | ± 120° |
| Ogologo oge | Linear |
| Oge mgbanwe | 50ms ruo 18h |
| Oge ịgba ọsọ kacha | 999h |
| Ọnụọgụ nke Electrodes | 24, na-achịkwa onwe ya |
| Oke okpomọkụ | 0℃ ruo 50℃, njehie nchọpụta <± 0.5℃ |
Nkọwa
Gel electrophoresis pulsed (PFGE) na-ekewa ụmụ irighiri DNA site na ịgbanye ọkụ eletrik n'etiti ụzọ abụọ eletrọnịkị oghere, na-eme ka mkpụrụ ndụ DNA, nke nwere ike ịbụ ọtụtụ nde ụzọ abụọ dị ogologo iji tụgharịa na ịkwaga site na pores gel agarose na ọsọ dị iche iche. Ọ na-enweta mkpebi dị elu n'ime oke a ma a na-ejikarị ya na usoro ihe ọmụmụ sịntetik; njirimara nke usoro ndu na microbial; nnyocha na molekul epidemiology; ọmụmụ nke nnukwu iberibe plasmid; localization nke mkpụrụ ndụ ihe nketa ọrịa; maapụ anụ ahụ nke mkpụrụ ndụ ihe nketa, nyocha RFLP, na akara mkpịsị aka DNA; nchọpụta ọnwụ cell emebere; ọmụmụ banyere mmebi na nrụzi DNA; ikewapụ na nyocha nke DNA genomic; nkewa nke chromosomal DNA; owuwu, njirimara, na nyocha nke nnukwu ọba akwụkwọ genomic; na transgenic research.t ntinye uche dị ka 0.5 ng/µL (dsDNA).
Ngwa
Kwesịrị ekwesị maka ịchọpụta na ikewapụta mkpụrụ ndụ DNA sitere na 100bp ruo 10Mb n'ogo, na-enweta mkpebi dị elu n'ime oke a.
Njirimara
• Teknụzụ dị elu: Na-ejikọta teknụzụ CHEF na PACE pulsed-field iji nweta nsonaazụ kacha mma na ụzọ kwụ ọtọ, anaghị agbagọ.
• Njikwa nọọrọ onwe ya: Atụmatụ 24 platinum electrodes na-achịkwa onwe ya (dayameta 0.5mm), na-eji electrode ọ bụla dochie ya n'otu n'otu.
• Ọrụ Mgbakọ akpaaka: Na-ejikọta ọtụtụ isi mgbanwe dị ka voltaji gradient, okpomọkụ, mgbanwe n'akuku, oge mbụ, oge njedebe, oge mgbanwe ugbu a, ngụkọta oge ịgba ọsọ, voltaji, na ugbu a maka mgbakọ na-akpaghị aka, na-enyere ndị ọrụ aka nweta ọnọdụ nnwale kacha mma.
• Algorithm Pụrụ Iche: Na-eji algọridim njikwa pulse pụrụ iche maka mmetụta nkewa ka mma, na-amata ọdịiche dị n'etiti linear na DNA okirikiri, yana nkwalite nkewa nke nnukwu DNA okirikiri.
• akpaaka: Na-edekọ na-akpaghị aka ma malitegharịa electrophoresis ma ọ bụrụ na a kwụsịrị usoro ahụ n'ihi ọdịda ike.
• Onye ọrụ-Configurable: Na-enye ndị ọrụ ohere ịtọ ọnọdụ nke ha.
• Mgbanwe: Sistemu nwere ike họrọ gradients voltaji akọwapụtara na oge ngbanwe maka oke nha DNA.
• Nnukwu ihuenyo: Ejiri ya na ihuenyo LCD 7-inch maka ịrụ ọrụ dị mfe, na-egosipụta njikwa ngwanrọ pụrụ iche maka iji dị mfe ma dị mma.
• Nchọpụta okpomoku: Nchọpụta okpomọkụ abụọ na-achọpụta kpọmkwem okpomọkụ na-echekwa ya na oke mperi na-erughị ± 0.5 ℃.
• Sistemụ okirikiri: na-abịa na sistemụ mgbasa ozi nke na-achịkwa nke ọma ma na-enyocha ọnọdụ okpomọkụ nke ihe nchekwa, na-eme ka ọnọdụ okpomọkụ na-adị mgbe niile na nguzozi ionic n'oge electrophoresis.
• Nchekwa dị elu: Gụnyere mkpuchi nchekwa acrylic transperent nke na-ebipụ ike na-akpaghị aka mgbe ebuliri ya, yana oke ibu na ọrụ nchebe enweghị ibu.
• Nhazi ọkwa: Tank electrophoresis na ihe nkedo gel na-egosipụta ụkwụ na-agbanwe agbanwe maka ọkwa.
• Ebube Ebube: A na-eji tankị electrophoresis na-emepụta ihe na-ejikọta ya na-enweghị njikọ; A na-eji igwe elektrọd platinum 0.5mm kwadoro ya, na-ahụ maka ịdịte aka na nsonaazụ nnwale kwụsiri ike.
FAQ
Ajụjụ: Gịnị bụ Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis bụ usoro eji kewapụta nnukwu mkpụrụ ndụ DNA dabere na nha ha. Ọ na-agụnye ịgbanye ntụziaka nke ọkụ eletrik na gel matrix iji mee ka ikewa nkewa nke DNA nke buru ibu ka a ga-edozi ya site na omenala agarose gel electrophoresis.
Q: Gịnị bụ ngwa nke Pulsed Field Gel Electrophoresis?
A: A na-eji Pulsed Field Gel Electrophoresis mee ihe na ihe ọmụmụ ihe omimi na mkpụrụ ndụ ihe nketa maka:
Nhazi nke ụmụ irighiri DNA buru ibu, dị ka chromosomes na plasmids.
• Ịchọpụta nha genome.
• Ịmụ ihe dị iche iche nke mkpụrụ ndụ ihe nketa na mmekọrịta evolushọn.
• Ọrịa na-efe efe molecular, ọkachasị maka nsochi ntiwapụ nke ọrịa na-efe efe.
• Nyocha nke mmebi na nrụzi DNA.
• Ịchọpụta ọnụnọ nke mkpụrụ ndụ ihe nketa ma ọ bụ usoro DNA.
Ajụjụ: Kedu ka Pulsed Field Gel Electrophoresis si arụ ọrụ?
A: Pulsed Field Gel Electrophoresis na-arụ ọrụ site n'itinye ụmụ irighiri DNA n'okirikiri ọkụ eletrik nke na-atụgharị na ntụziaka. Nke a na-enye ohere ka ụmụ irighiri DNA buru ibu gbanwee onwe ha n'etiti pulses, na-eme ka mmegharị ha site na gel matrix. Mkpụrụ ndụ DNA pere mpe na-aga ngwa ngwa site na gel, ebe ndị buru ibu na-eji nwayọ nwayọ, na-enye ohere nkewa ha dabere na nha.
Ajụjụ: Gịnị bụ ụkpụrụ n'azụ Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis na-ekewa mkpụrụ ndụ DNA dabere na nha ha site n'ịchịkwa oge na ntụziaka nke ọkụ eletrik. Ebe a na-agbanwe agbanwe na-eme ka mkpụrụ ndụ DNA buru ibu na-emegharị onwe ha mgbe niile, na-eduga na njem ha site na gel matrix na nkewa dịka nha.
Q: Gịnị bụ uru nke Pulsed Field Gel Electrophoresis?
A: Mkpebi dị elu maka ikewapụta mkpụrụ ndụ DNA buru ibu ruo ọtụtụ nde isi ụzọ abụọ. Ikike iji dozie na ịmata ọdịiche dị iche iche DNA nke nha nha nha.Versatility na ngwa ngwa, site na ntinye microbial na mkpụrụ ndụ ihe nketa molecular na genomics.Eguzobere usoro maka ọmụmụ ihe gbasara ọrịa na mkpụrụ ndụ ihe nketa.
Ajụjụ: Kedu akụrụngwa achọrọ maka Pulsed Field Gel Electrophoresis?
A: pulsed Field Gel Electrophoresis na-achọkarị ngwa electrophoresis nwere electrodes pụrụ iche maka ịmepụta ubi ndị a na-amịkọrọ. Agarose gel matrix nwere ntinye uche na nchekwa kwesịrị ekwesị. Ike ọkọnọ ike nke na-amụba elu-voltage pulses.Cooling usoro ikposa okpomọkụ eme n'oge electrophoresis, na mgbasa ozi mgbapụta.
