Electrophoresis Nyefee niile-na-otu Sistemụ
Nkọwapụta
| Nkọwapụta maka Electrophoresis Tank | |
| Nha gel (LxW) | 83×73mm |
| Ngwakọta | olulu mmiri iri (Standard) olulu mmiri iri na ise (nhọrọ) |
| Ọkpụrụkpụ Comb | 1.0 mm (Ọkọlọtọ) 0.75, 1.5 mm (Nhọrọ) |
| Mpekere iko dị mkpụmkpụ | 101 × 73mm |
| Spacer Glass Plate | 101 × 82mm |
| Olu ihe nchekwa | 300 ml |
| Nkọwapụta maka Modul Nyefee | |
| Mpaghara mkpochapụ (LxW) | 100 × 75mm |
| Ọnụọgụ nke Gel | 2 |
| Ogologo Electrode | 4cm |
| Olu ihe nchekwa | 1200ml |
| Nkọwapụta maka ikenye ike electrophoresis | |
| Akụkụ (LxWxH) | 315 x 290 x 128mm |
| Voltaji mmepụta | 6-600V |
| Mmepụta ugbu a | 4-400mA |
| Ike mmepụta | 240W |
| Ọnụ ahịa mmepụta | 4 ụzọ abụọ n'otu n'otu |
Nkọwa
Usoro ihe niile na-enyefe electrophoresis na-agụnye tankị electrophoresis na mkpuchi, ọkụ ọkụ na njikwa njikwa, na modul nyefe na electrodes. A na-eji tankị electrophoresis mee ihe nkedo ma na-agba ọsọ gels, a na-ejikwa modul nyefe na-ejide gel na akpụkpọ anụ Sanwichi n'oge usoro mbufe, ọ nwekwara igbe dị jụụ iji gbochie ikpo ọkụ. Ọkụ ọkụ na-enye ọkụ eletrik dị mkpa iji na-agba ọsọ gel ma na-ebugharị nnyefe nke ụmụ irighiri ihe site na gel na akpụkpọ ahụ, ma nwee njikwa njikwa enyi na enyi maka ịtọ ntọala electrophoresis na ọnọdụ mbufe. Modul mbufe na-agụnye electrodes nke etinyere na tank wee bata na gel na akpụkpọ ahụ, na-emecha sekit eletriki achọrọ maka ịnyefe.
The electrophoresis na-ebufe niile na-na-otu bụ ngwá ọrụ dị mkpa maka ndị na-eme nchọpụta na technicians na-arụ ọrụ na protein samples. Nhazi ya na ịdị mfe iji ya na-eme ka ọ bụrụ mgbakwunye bara uru na ụlọ nyocha ọ bụla na-etinye aka na bayoloji molecular ma ọ bụ nyocha biochemistry.
Ngwa
Usoro ihe niile na-enyefe electrophoresis bụ ngwá ọrụ bara uru n'ọhịa nke bayoloji molecular, kpọmkwem na nyocha protein. A na-achọpụtakwa protein ndị ebufetara site na iji ọgwụ mgbochi ụfọdụ na usoro akpọrọ Western blotting. Usoro a na-enye ndị na-eme nchọpụta aka ịchọpụta kpọmkwem protein ndị nwere mmasị na ọnụ ọgụgụ okwu ha.
Egosiputara
• Ngwaahịadabara maka obere nha PAGE gel electrophoresis;
• Ngwaahịa's parameters, ngwa na-n'ụzọ zuru ezu dakọtara na isi ika ngwaahịa na ahịa;
•Ọdịdị dị elu na nhazi siri ike;
• Gbaa mbọ hụ na nsonaazụ nnwale dị mma site na nkedo gel na-agba ọsọ;
•Ngwa ngwa nyefee obere gels;
• Enwere ike itinye cassettes abụọ nke gel na tank;
• Nwere ike ịgba ọsọ ruo 2 gel n'ime otu awa. Ọ nwere ike na-arụ ọrụ n'ime abalị maka nnyefe dị ala;
• Cassettes njide jel nwere agba dị iche iche na-eme ka ọ bụrụ nke ziri ezi.
FAQ
Ajụjụ: Gịnị bụ electrophoresis nyefe niile-na-otu usoro eji?
A: A na-eji usoro ihe niile na-enyefe electrophoresis maka ịnyefe protein site na gel polyacrylamide na akpụkpọ ahụ maka nyocha ọzọ, dị ka Western blotting.
Ajụjụ: Gịnị bụ nha nke gel nke nwere ike ime ma bufee site na iji electrophoresis na-ebufe ihe niile n'ime otu?
A: electrophoresis na-enyefe ihe niile n'ime otu usoro nwere ike ịtụba ma mee nha jel 83X73cm maka nkedo aka, yana 86X68cm nkedo nkedo. Ebe mbufe bụ 100X75cm.
Ajụjụ: Kedu ka electrophoresis na-ebufe ihe niile na-arụ ọrụ?
A: The electrophoresis nyefee niile-na-otu usoro na-eji electrophoresis nyefee protein si jel na akpụkpọ ahụ. A na-ebu ụzọ kewaa protein ndị ahụ site na nha site na iji polyacrylamide gel electrophoresis (PAGE) wee bufee ya na akpụkpọ ahụ site na iji igwe eletrik.
Ajụjụ: Kedu ụdị akpụkpọ anụ nwere ike iji na electrophoresis na-ebufe ihe niile n'ime otu?
A: Enwere ike iji ụdị akpụkpọ anụ dị iche iche na electrophoresis na-ebufe usoro ihe niile gụnyere nitrocellulose na PVDF (polyvinylidene difluoride).
Ajụjụ: Enwere ike iji usoro ihe niile na-enyefe electrophoresis maka nyocha DNA?
A: Mba, A na-emepụta usoro ihe niile na-enyefe electrophoresis kpọmkwem maka nyocha protein na enweghị ike iji ya mee ihe maka nyocha DNA.
Ajụjụ: Gịnị bụ uru nke iji electrophoresis nyefe niile-na-otu usoro?
A: Igwe electrophoresis na-enyefe ihe niile na-enye ohere maka ịnyefe protein nke ọma site na gel na akpụkpọ anụ, na-enye nnukwu uche na nkọwapụta na nchọpụta protein. Ọ bụkwa usoro dabara adaba nke na-eme ka usoro mkpochapụ Western dị mfe.
Ajụjụ: Olee otú e kwesịrị isi na-edobe electrophoresis nyefe niile-na-otu?
A: A na-ehichapụ ihe niile na-enyefe electrophoresis mgbe ejiri ya mee ihe ma chekwaa ya na ebe dị ọcha. Ekwesịrị ka a na-enyocha elektrọd na akụkụ ndị ọzọ mgbe niile maka mmebi ma ọ bụ iyi na akwa.
