Cellulose acetate membrane Electrophoresis

Kedu ihe bụ Cellulose Acetate Membrane Electrophoresis?

Cellulose Acetate Membrane Electrophoresis bụ otu ụdị usoro electrophoresis nke na-eji cellulose acetate membrane dị ka mgbasa ozi na-akwado maka nnwale.

Cellulose acetate bụ ụdị acetate nke cellulose nke acetylated sitere na cellulose's hydroxyl.Mgbe ọ bụ soluble na organic ngwọta dị ka acetone, ọ nwere ike kpuchie ya na homogeneous na mma film na micro-pores.Ihe nkiri ahụ dị gburugburu 0.1-0.15mm nke kwesịrị ekwesị maka nnwale.

Uru nke cellulose acetate membrane

N'iji ya tụnyere akwụkwọ fliter, ọ nwere uru ndị a:

1.Good mmetụta nkewa.Maka ihe nlele protein, akpụkpọ anụ acetate cellulose na-amịkọrọ ole na ole n'ime ha, mgbe agbachara, ọ nwere ike ịchacha kpamkpam site na agba agba.Ngwakọta agba agba a doro anya nke ọma, ya mere ọ na-akwalite izi ezi maka ikpebi ọnụọgụgụ.
2. Fast na-azọpụta oge.Akpụkpọ anụ acetate cellulose dị obere hydyophilic karịa akwụkwọ fliter, yabụ arụmọrụ electro-osmosis dị obere karịa akwụkwọ fliter, na ọkụ eletrik na-abụkarị ihe nlele nke nwere ike belata oge nkewa ma belata oge electrophoresis.Dị ka ọ na-adịkarị, cellulose acetate membrane electrophoresis oge bụ ihe dị ka nkeji 45-60.Usoro electrophoresis dum gụnyere ịjiji na decoloration chọrọ naanị nkeji 90.
3.High sensitivity na obere samples ga-eji.Ọ chọrọ naanị 2 μL serum na serum protein electrophoresis.
4.Wide ngwa.Ụfọdụ protein adịghị mfe ikewapụ na akwụkwọ electrophoresis, dị ka Alpha-fetoprotein, bacteriolytic enzyme, insulin, na histone wdg Ma ọ ka mma iji cellulose acetate membrane electrophoresis ikewapụ ha.
5.Easy idebe na quantification.Mgbe agbachara ihe nlele nke cellulose acetate membrane electrophoresis, gbanye ihe nlele ahụ n'ime ngwakọta agwakọta nke glacial acetic acid na ethanol iji mee ka efere akọrọ na-apụta ìhè nke echekwara ogologo oge.

N'ihi na cellulose acetate membrane electrophoresis dị mfe ịrụ ọrụ, ugbu a, a na-eji ya eme ihe na nyocha na nyocha protein protein plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid na ndị ọzọ biomacromolecule.

Nkwadebe maka Cellulose Acetate Membrane Electrophoresis

Ngwa eji eme ihe na-agụnye ọkụ eletrik dị ala, tank electrophoresis kwụ ọtọ.Mgbasa mgbasa ozi na-akwado bụ cellulose acetate membrane.

Ndị reagents:

1.pH8.6 barbitol ngwọta ngwọta: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain: Ponceau S, Trichloroacetic;
3.TBS / T ma ọ bụ PBS / T: 95% ethanol, glacial acetic acid;
4.Cleaning Solution: anhydrous ethanol, glacial acetic acid.

Beijing Liuyi Biotechnology Co., Ltd nwere usoro tozuru okè maka cellulose acetate membrane electrophoresis.Ihe nlereanya DYCP-38C bụ ụlọ dị nro maka akwụkwọ na slide electrophoresis, yana cellulose acetate membrane electrophoresis.A na-akpụzi ahụ isi, ọ nweghị ihe na-agbapụta.Ụdị ọkụ ọkụ DYY-2C, DYY-6C, DYY-6D, DYY-8C , DYY-10C na DYY-12 nwere ike ịnye ike maka DYCP-38C.N'ozuzu, onye ahịa na-enwe mmasị ịhọrọ ụdị DYY-6C dị ka ọkụ ọkụ maka DYCP-38C.

Dị ka ngwaahịa dị mkpa maka DYCP-38C electrophoresis tank, Liuyi na-enyekwa cellulose acetate akpụkpọ ahụ.Enwere nha atọ nke akpụkpọ ahụ, 7 * 9cm, 2 * 8cm na 12 * 8cm, anyị nwekwara ike ịnye nha nke cellulose acetate akpụkpọ anụ ahaziri dịka ihe gị chọrọ.

Ihe ndetu gbasara nnwale:

1. Ibunye ihe nlele na akụkụ siri ike: 1-2UL dabara adaba
2.Constant ugbu a electrophoresis: ike ugbu a 0.4 ~ 0.6m A / cm
3.pH8.6 barbitol nchekwa ihe ngwọta: ike ionic 0.06
4.Dyeing oge: 5 nkeji ezuru
5.Preservation: tinye map elecrtophoresis akọrọ na ngwọta nhicha maka 10-15 nkeji, wee wepụ ya ma tinye ya na iko dị ọcha, mgbe ọ nwụsịrị, ọ ga-aghọ map ihe nkiri transperent.

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