Ihe gbasara eriri protein electrophoresis na-ezo aka na nsogbu ma ọ bụ mmebi iwu nwere ike ibilite n'oge usoro nkewapụ protein dabere na ụgwọ eletrik ha site na iji electrophoresis.Okwu ndị a nwere ike ịgụnye ọdịdị nke ụgbụ a na-atụghị anya ya ma ọ bụ nke na-adịghị mma, mkpebi na-adịghị mma, mmetọ, ma ọ bụ mgbagha nke eriri protein, na ndị ọzọ.Anyị na-achịkọta ọtụtụ okwu ndị a na-ahụkarị maka ndị ahịa anyị na-ekwu maka ịchọpụta ihe kpatara ya na iji nweta nsonaazụ ziri ezi ma melite ogo nkewa protein.
mụmụọ ọnụ ọchịband- usoro eriri na-agbago elu n'akụkụ abụọ nke gel
Ihe kpatara ya
1. Ebe etiti gel na-agba ọsọ karịa njedebe ọ bụla
2. Ọnọdụ ike karịrị oke
Ngwọta
① Nchekwa adịghị agwakọta nke ọma ma ọ bụ ihe nchekwa n'ime ụlọ elu gbadoro anya nke ukwuu.Megharịa ihe nchekwa, na-ahụ na ngwakọta nke ọma, ọkachasị mgbe ị na-agbaze ngwaahịa 5x ma ọ bụ 10x
② Belata ntọala ike site na 200 V ruo 150 V ma ọ bụ jupụta ọnụ ụlọ dị n'ime 1 cm nke elu nke obere efere.
Ịgbasa protein kwụ ọtọ
Ihe kpatara ya
1. Sample ibubigara ókè
2. Ngwọta mmiri ozuzo sample
Ngwọta
① Na-atụgharị ihe nlele, họrọ nke ọma wepụ protein kachasị na nlele, ma ọ bụ belata voltaji ihe dịka 25% iji belata streaking.
② Ihe nlele centrifuge tupu mgbakwunye nke ihe nchekwa SDS, ma ọ bụ belata% T nke gel
③ Oke nke SDS na protein kwesịrị ezuru iji kpuchie molekul protein ọ bụla na SDS, n'ozuzu 1.4: 1.Ọ nwere ike ịchọkwu SDS maka ụfọdụ ihe atụ protein akpụkpọ anụ
Bnakehoraizinna-agbasa
Ihe kpatara ya
1. Mgbasa nke olulu mmiri tupu ịgbanye ugbu a
2. Ionic ike nke ihe nlele dị ala karịa nke gel
Ngwọta
① Wedata oge n'etiti ngwa nlele na ịgbanwuo mmalite ike
② Jiri otu ihe nchekwa dị na nlele dị ka ọ dị na gel ma ọ bụ gel stacking
Njikọ protein gbagọrọ agbagọ ma ọ bụ gbagharịa
Ihe kpatara ya
1. polymerization na-adịghị mma gburugburu olulu mmiri
2. Nnu na sample
3. Gel interface na-enweghị isi
Ngwọta
① Degas stacking gel ngwọta kpamkpam tupu nkedo;mụbaa ammonium persulfate na TEMED mkpokọta site 25%, maka stacking gel ma ọ bụ obere% T, hapụ APS otu na okpukpu abụọ nke TEMED itinye uche.
② Wepu nnu site na dialysis, desalting;
③ Wedata ọnụego polymerization.Machie gels nke ọma.
Beijing Liuyi Biotechnology Company Ltd na-arụpụta ọtụtụ ngwaahịa electrophoresis nke nwere ike inye aka dozie nsogbu ndị anyị nwere ike izute na nnwale electrophoresis anyị.
Beijing Liuyi Biotechnology Company Ltd hiwere na 1970, nke a na-akpọbu Beijing Liuyi Instrument Factory, bụ onye nrụpụta akụrụngwa ụlọ nyocha na ngwa sayensị dabere na Beijing, China.Na n'elu 50 afọ ahụmahụ na emebe na n'ichepụta ngwaahịa maka electrophoresis na ndụ sayensị nnyocha, ọ na-aghọ a na-eduga na-eweta nke laabu ngwá na ngwọta na China.The ụlọ ọrụ ngwaahịa na-agụnye a dịgasị iche iche nke laabu ngwá, gụnyere kehoraizin nucleic acid electrophoresis tank, vetikal protein. tank electrophoresis / unit, igbe ojii ụdị UV analyzer, Gel Document Tracking Imaging Analyzer, na electrophoresis ike ọkọnọ.A na-eji ngwaahịa ndị a eme ihe n'ọtụtụ ebe na ụlọ ọrụ nyocha, mahadum, na ụlọ ọrụ dị iche iche, dị ka ọgwụ, nkà na ụzụ, na nlekota gburugburu ebe obibi. Ụlọ ọrụ ahụ bụ ISO9001 & ISO13485 ụlọ ọrụ kwadoro ma nwee asambodo CE.
Enwereụdị dị iche ichevetikalelectrophoresis tank makaelectrophoresis proteinmaka nyocha na njirimara nke ihe atụ protein site na polyacrylamide gel electrophoresis,nanakwa maka ịlele sample 'molecular arọ, ọcha samples na-akwadebe samples.A na-anabata ngwaahịa ndị a niileahịa ụlọ na esenidụt.
The electrophoresis ike ọkọnọ bụ ihe dị mkpa akụrụngwa nkeusoro nke electrophoresis, na-enye ebe ọkụ eletrik kwụ ọtọ na nke ziri ezi iji mee ka usoro nkewa ahụ dị.Itna-ebunye voltaji na-adịgide adịgide ma ọ bụ ugbu a na sistemụ electrophoresis, dabere na ụkpụrụ nnwale akọwapụtara.Ọ na-enye ohere ka onye ọrụ gbanwee voltaji ma ọ bụ mmepụta ugbu a, yana ihe ndị ọzọ dị ka oge na okpomọkụ, iji kwalite ọnọdụ nkewa maka otu nnwale.
Fma ọ bụ na-ekiri gel, ị nwere ike họrọ UV Transilluminator WD-9403 usoro nke Beijing Liuyi Biotechnology rụpụtara.A UV transilluminator bụ ngwa ụlọ nyocha eji elele anya yana nyocha DNA, RNA na protin.Ọ na-arụ ọrụ site na iji ọkụ UV na-amụba ihe atụ ndị ahụ, nke na-eme ka ihe nlele ahụ na-egbuke egbuke ma bụrụ ndị a na-ahụ anya.Enwere ọtụtụ ụdị nke transilluminator UVnke anyị nyere gị.WD-9403A bụ nke pụrụ iche maka ịhụ protein electrophoresis, na WD-9403F na-eji maka ịhụ DNA na protein electrophoresis.
Usoro ngwaahịa ndị a nwere ike ije ozi site na nkedo gel ruo na-elele gel dịka ihe nnwale gị siri dị.Mee ka anyị mara ihe ị chọrọ, ma OEM, ODM na ndị nkesa anabatara.We ga-agbalị ike anyị niile na-enye gị ngwaahịa na ọrụ anyị.
Ọ bụrụ na ị nwere atụmatụ ịzụta maka ngwaahịa anyị, biko egbula oge ịkpọtụrụ anyị.Ị nwere ike izipu anyị ozi na email[email na-echebe]ma ọ bụ[email na-echebe], ma ọ bụ biko kpọọ anyị na +86 15810650221 ma ọ bụ tinye Whatsapp +86 15810650221, ma ọ bụ Wechat: 15810650221.
Oge nzipu: Mar-29-2023